Temperature-sensitive mutant in the vaccinia virus E6 protein produce virions that are transcriptionally inactive.
Temperature-sensitive mutant in the vaccinia virus E6 protein produce virions that are transcriptionally inactive.
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DOI：
10.1016/j.virol.2010.01.010
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发表时间：
2010-04-10
期刊：
影响因子：
3.7
通讯作者：
Moussatche N
中科院分区：
文献类型：
--
作者：
Boyd O;Strahl AL;Rodeffer C;Condit RC;Moussatche N
The vaccinia virus E6R gene encodes a late protein that is packaged into virion cores. A temperature sensitive mutant was used to study the role of this protein in viral replicative cycle. Cts52 has a P226L missense mutation in the E6R gene, shows a two-log reduction in plaque formation, but displays normal patterns of gene expression, late protein processing and DNA replication during infection. Mutant virions produced at 40°C were similar in their morphology to wt virions grown at 40°C. The particle to infectivity ratio was 50 times higher in purified Cts52 grown at 40°C when compared to the mutant grown at permissive temperature. In vitro characterization of Cts-52 particles grown at 40 °C revealed no differences in protein composition or in DNA content and the mutant virions could bind and enter cells. However, core particles prepared from Cts52 grown at 40 °C failed to transcribe in vitro. Our results show that E6 in the virion has either a direct or an indirect role in viral transcription.
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影响因子：
5.4
作者：
Hu, XL;Carroll, LJ;Moss, B
通讯作者：
Moss, B
影响因子：
3.7
作者：
Kato, Sayuri E. M.;Condit, Richard C.;Moussatche, Nissin
通讯作者：
Moussatche, Nissin
影响因子：
5.4
作者：
CARPENTER, MS;DELANGE, AM
通讯作者：
DELANGE, AM
影响因子：
5.4
作者：
ERICSSON, M;CUDMORE, S;LOCKER, JK
通讯作者：
LOCKER, JK
DOI：
10.1083/jcb.10.4.475
发表时间：
1961-08
期刊：
The Journal of biophysical and biochemical cytology
影响因子：
--
作者：
DALES, S;SIMINOVITCH, L
通讯作者：
SIMINOVITCH, L