Frequency of antibodies to the cholesterol transport protein apolipoprotein A1 in patients with SLE
Frequency of antibodies to the cholesterol transport protein apolipoprotein A1 in patients with SLE
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DOI：
10.1191/096120398678920262
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发表时间：
1998-01-01
期刊：
影响因子：
2.6
通讯作者：
Lahita, RG
中科院分区：
文献类型：
--
作者：
Dinu, AR;Merrill, JT;Lahita, RG
在研究系统性红斑狼疮(SLE)早期动脉粥样硬化的原因时，我们先前报道在这些患者中胆固醇转运蛋白apoA1(ApoA1)水平较低，并且在30例狼疮患者中有5例的血清中发现了针对纯化apoA1的特异性抗体。目前的研究是为了确定这些抗体在狼疮患者中是否常见。对175例系统性红斑狼疮(SLE)或原发性抗磷脂综合征(PAPS)患者的520份血清标本进行了纯化载脂蛋白A1抗体检测。重新检测阳性血清与重组的新生或成熟高密度脂蛋白(HDL)结合的载脂蛋白。自身抗体TC、apoA1在32.5%的SLE和22.9%的PAPS患者中出现，且与APL(抗β(2)糖蛋白-1、抗β(2)GPL)抗体的存在有关。将重建的、新生的和天然的高密度脂蛋白分子作为抗原环境进行比较，阳性血清与成熟高密度脂蛋白分子包埋的载脂蛋白A1反应最好。这份报告证实了系统性红斑狼疮患者中apoA1抗体的高流行率，并提示这些抗体与成熟的高密度脂蛋白有很高的亲和力。
In examining reasons for premature atherosclerosis in systemic lupus erythematosus (SLE), we previously reported low levels of the cholesterol transport protein apolipoprotein Al (apoAl) in these patients, and specific antibodies to purified apoAl were identified in the sera of 5 out of 30 lupus patients. The current study was initiated to determine whether these antibodies are common in lupus patients. 520 serum samples from 175 patients with SLE or primary antiphospholipid syndrome (PAPS) were tested for antibodies to purified apoAl. Positive sera were retested for binding to apolipoprotein incorporated into reconstructed nascent or mature high-density lipoprotein (HDL). Autoantibodies tc, apoAl were found in 32.5% of patients with SLE and 22.9% of patients with PAPS, associated with the presence of aPL (anti-beta(2) glycoprotein-1, anti beta(2) GPl) antibodies. When reconstructed, nascent and nature HDL molecules were compared as antigen-containing environments, positive sera reacted best to apoAl embedded in mature HDL molecules. This report confirms the high prevalence of antibodies to apoAl in patients with systemic lupus and suggests a high affinity of these antibodies for mature HDL.