Erythromycin and spiramycin resistance mutations of yeast mitochondria: nature of the rib2 locus in the large ribosomal RNA gene.
Erythromycin and spiramycin resistance mutations of yeast mitochondria: nature of the rib2 locus in the large ribosomal RNA gene.
复制标题
酵母线粒体的红霉素和螺旋霉素抗性突变：大核糖体 RNA 基因中 rib2 基因座的性质。
作者：
F. Sor;H. Fukuhara
酵母线粒体基因组的两个相连的基因座，肋2和肋3，是产生对红霉素和/或螺旋霉素耐药的突变位点。我们已经检查了肋骨2位点的两个突变。突变ER354位于大核糖体RNA基因的核苷酸位置3993；它对应于C到G的转化，导致对红霉素和螺旋霉素的双重耐药。在同一位置也发现了突变SR551，但C被T取代，仅赋予对螺旋霉素的抗性。肋骨2和肋骨3在基因序列上相距836个碱基对，但在核糖体RNA的二级结构上彼此非常接近。
Two linked genetic loci, rib 2 and rib 3, of yeast mitochondrial genome are the sites of mutations that confer resistance to erythromycin and/or spiramycin. We have examined two mutations at the rib 2 locus. Mutation ER354 was found at the nucleotide position 3993 of the large ribosomal RNA gene; it corresponded to a C to G transversion leading to a double resistance to erythromycin and spiramycin. Mutation SR551 was found also at the same position, but the C was replaced by a T, conferring resistance to spiramycin only. Rib 2 and rib 3 are 836 base pairs apart on the gene sequence, but are very close to each other in the secondary structure of ribosomal RNA.