Cellular Origin of Testis-Derived Pluripotent Stem Cells: A Case for Very Small Embryonic-Like Stem Cells
Cellular Origin of Testis-Derived Pluripotent Stem Cells: A Case for Very Small Embryonic-Like Stem Cells
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DOI：
10.1089/scd.2011.0554
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发表时间：
2012-03-01
影响因子：
4
通讯作者：
Shaikh, Ambreen
中科院分区：
文献类型：
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作者：
Bhartiya, Deepa;Kasiviswananthan, Sandhya;Shaikh, Ambreen
有人认为睾丸生殖干细胞代表了唯一的成体干细胞，去分化和重编程成多能状态，没有任何遗传修饰。关于源自成人睾丸组织的胚胎干细胞（ES）样细胞的真实性的争论促使我们提出这封信。我们希望加强我们的发现，多能性非常小的胚胎样干细胞（VSELs）存在于成年哺乳动物睾丸中的一个小群体，并可能导致ES样集落。由于它们的小尺寸，感觉VSEL可能污染用于接种的初始细胞，尽管努力将单个生殖细胞置于96孔板中用于克隆扩增，或使用磁性激活细胞分选（MACS）分选的α 6整联蛋白阳性细胞。类似地，感觉到VSEL与间充质干细胞（MSC）一起沿着存在于各种组织中可以为MSC的转分化潜力提供替代解释。我们的结论是，像Oct-4生物学一样，VSELs在成人身体组织中的存在让干细胞生物学家感到有些惊讶。
It has been suggested that testicular germ stem cells represent the only adult body stem cells that dedifferentiate and reprogram into a pluripotent state without any genetic modification. Emerging debate about the authenticity of embryonic stem cell (ES)-like cells derived from adult testicular tissue has prompted us to put forth this letter. We wish to reinforce our findings that pluripotent very small embryonic-like stem cells (VSELs) exist as a small population in adult mammalian testis and may result in ES-like colonies. Because of their small size, it is felt that VSELs could be contaminating the initial cells used for seeding, although efforts were made to place a single germ cell per well in a 96-well plate for clonal expansion, or magnetic activated cell sorting (MACS)-sorted alpha 6 integrin positive cells were used. On a similar note, it is felt that the presence of VSELs in various tissues along with mesenchymal stem cells (MSCs) may provide an alternative explanation to the transdifferentiation potential of MSCs. We conclude that like Oct-4 biology, presence of VSELs in adult body tissues has somewhat surprised stem cell biologists.