Dietary Tangeretin Alleviated Dextran Sulfate Sodium-Induced Colitis in Mice via Inhibiting Inflammatory Response, Restoring Intestinal Barrier Function, and Modulating Gut Microbiota
Dietary Tangeretin Alleviated Dextran Sulfate Sodium-Induced Colitis in Mice via Inhibiting Inflammatory Response, Restoring Intestinal Barrier Function, and Modulating Gut Microbiota
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膳食橘皮素通过抑制炎症反应、恢复肠道屏障功能和调节肠道微生物群减轻小鼠硫酸葡聚糖钠诱导的结肠炎
DOI：
10.1021/acs.jafc.1c03046
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发表时间：
2021-06-29
影响因子：
6.1
通讯作者：
Song, Mingyue
中科院分区：
文献类型：
--
作者：
Chen, Bin;Luo, Jiakang;Song, Mingyue
本研究观察了柑橘类天然类黄酮衍生物--橘皮黄素(Tan)对葡聚糖硫酸钠(DSS)诱导的小鼠结肠炎的预防作用。结果表明，日粮中添加0.04%和0.08%的丹参酮(0.04%和0.08%w/w)可显著减轻DSS引起的小鼠结肠炎的严重程度，表现为增加了小鼠的结肠长度，降低了疾病活动性指数，减轻了结肠组织的损伤。此外，膳食Tan通过下调结肠炎性细胞因子的过度表达来抑制炎症反应。免疫组织化学分析显示，丹参通过上调Claudin-1和ZO-1的表达，恢复肠屏障功能。此外，膳食Tan通过提高肠道微生物区系多样性，提高有益细菌(如乳杆菌科和乳杆菌科)的水平，降低有害细菌(如肠杆菌科和泽泻属)的水平，来调节结肠炎小鼠的肠道微生物区系。此外，饮食中的丹参促进了DSS处理小鼠的短链脂肪酸的产生。总之，这些发现为合理利用丹参作为结肠炎和相关疾病的预防药物提供了科学依据。
In this study, the preventive effect of tangeretin (TAN), a natural flavonoid derivative from citrus fruits, on the dextran sulfate sodium (DSS)-induced colitis in mice was evaluated. Our results showed that dietary TAN (0.04% and 0.08% w/w in the diet) significantly reduced the severity of colitis caused by DSS treatment in mice, evidenced by the increased colon length, the reduced disease activity index, and the attenuated colonic tissue damages. Moreover, dietary TAN inhibited the inflammatory response via down-regulating the overexpression of colonic inflammatory cytokines. Immunohistochemical analysis revealed that the intestinal barrier function was restored by TAN through enhancing claudin-1 and ZO-1 expressions. Additionally, dietary TAN modulated gut microbiota in colitic mice via enhancing gut microbiota diversity, ascending the level of beneficial bacteria, e.g., Lachnospiraceae and Lactobacillaceae, and descending the level of harmful bacteria, e.g., Enterobacteriaceae and Alistipes. Besides, dietary TAN promoted short-chain fatty acids production in DSS-treated mice. Altogether, these findings provided scientific evidence for the rational utilization of TAN as a preventive agent against colonic inflammation and related diseases.