New insights into the mechanical properties of Acanthamoeba castellanii cysts as revealed by phonon microscopy
New insights into the mechanical properties of Acanthamoeba castellanii cysts as revealed by phonon microscopy
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DOI：
10.1364/boe.10.002399
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发表时间：
2019-05-01
影响因子：
3.4
通讯作者：
Clark, Matt
中科院分区：
文献类型：
--
作者：
Perez-Cota, Fernando;Smith, Richard J.;Clark, Matt
单细胞真核原生动物卡氏棘阿米巴表现出从营养滋养体阶段转变为囊状阶段的非凡能力，以响应压力。这种表型转换涉及细胞壁的基因表达和合成的变化，这会影响生物体抵抗杀生剂和化疗药物的能力。鉴于包囊是卡氏拟线虫生活史中的一种基本生存机制，了解这一过程应该具有重要的环境和医学意义。在本研究中，我们使用一种新的声子显微镜技术在单细胞水平上研究了卡氏曲霉的包囊机制。声子显微镜是一种使用激光产生的亚光波声子对细胞成像的新兴技术。这种成像方式可以在光学或更高分辨率下以细胞的机械属性支持的对比度成像。我们的结果表明，布里渊频率，即由声子引起的光的颜色的移动，在三个明确定义的频带内演化，而不是简单的频率移动。这些观察结果证实了先前文献中的结果，并为卡氏锥虫包囊在恶劣环境中快速反应的能力提供了新的见解。由光学学会根据知识共享署名4.0许可证的条款出版。
The single cell eukaryotic protozoan Acanthamoeba castellanii exhibits a remarkable ability to switch from a vegetative trophozoite stage to a cystic form, in response to stressors. This phenotypic switch involves changes in gene expression and synthesis of the cell wall, which affects the ability of the organism to resist biocides and chemotherapeutic medicines. Given that encystation is a fundamental survival mechanism in the life cycle of A. castellanii, understanding of this process should have significant environmental and medical implications. In the present study, we investigated the mechanism of A. castellanii encystation using a novel phonon microscopy technique at the single cell level. Phonon microscopy is an emerging technique to image cells using laser-generated sub-optical wavelength phonons. This imaging modality can image with contrast underpinned by mechanical properties of cells at an optical or higher resolution. Our results show that the Brillouin frequency, a shift of the colour of light induced by phonons, evolves in three well defined frequency bands instead of a simple shift in frequency. These observations confirm previous results from literature and provide new insights into the capacity of A. castellanii cyst to react quickly in harsh environments. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.