Oral Lactobacilli Related to Caries Status of Children with Primary Dentition
Oral Lactobacilli Related to Caries Status of Children with Primary Dentition
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DOI：
10.1159/000506468
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发表时间：
2020-08-01
期刊：
影响因子：
4.2
通讯作者：
Matsumoto-Nakano, Michiyo
中科院分区：
文献类型：
--
作者：
Lapirattanakul, Jinthana;Nomura, Ryota;Matsumoto-Nakano, Michiyo
口腔乳酸杆菌是一组与龋齿进展有关的细菌的成员，尽管关于它们的传播、定植和龋齿相关物种的信息还没有很好地建立。本研究从乳牙期儿童口腔中分离乳酸杆菌，以了解乳牙期儿童口腔中乳酸杆菌的流行情况，并对乳牙期儿童口腔中致龋的主要致病菌--变形链球菌进行检测。通过16 S rDNA序列测定确定了分离的乳酸杆菌的种类。随后，最普遍的物种参与龋齿状态进行了评估，并确定与龋齿状态的allLactobacillusisolates的I型胶原蛋白的结合能力。采用11个位点的多位点序列分型（MLST）技术对优势乳杆菌菌株进行了研究。口腔乳酸杆菌与S.变形杆菌与最高的龋齿指数显著相关，但乳酸杆菌的胶原结合特性与龋齿状态无关，发酵乳酸杆菌是最普遍的，其存在与龋齿指数的高分有关。MLST分析L. fermentumpopulation不能指定与龋齿状态相关的特定克隆，但显示共享相同的L。在同一个教室里的孩子们之间发酵。总而言之，这些数据为口腔乳杆菌（尤其是L）的作用提供了有用的信息。龋齿发酵素
Oral lactobacilli are members of a group of bacteria implicated in caries progression, although information regarding their transmission, colonization, and caries-associated species is not well established. This study isolated oral lactobacilli from a group of children with primary dentition for determination ofLactobacillusprevalence, detection ofStreptococcus mutans, a major pathogen of caries initiation, and dental caries status of the children. Species ofLactobacillusisolates were determined from examination of 16S rDNA sequences. Subsequently, the most prevalent species was evaluated for involvement in caries status, and binding ability to type I collagen of allLactobacillusisolates was determined in association with caries status. Multilocus sequence typing (MLST) of eleven loci was carried out to study strains of the predominantLactobacillussp. The detection of oral lactobacilli together withS. mutanswas significantly associated with the highest dental caries indices, but there was no involvement of collagen-binding properties ofLactobacillusisolates in caries status.Lactobacillus fermentumwas the most prevalent, and its presence was related to high scores of caries indices. MLST analysis ofL. fermentumpopulation could not specify a particular clone associated with caries status, but revealed sharing of identicalL. fermentumstrains among children in the same classrooms. Taken together, the data contributed useful information on the role of oral lactobacilli, in particularL. fermentumin dental caries.