Separation of naphthalene-2,3-dicarboxaldehyde-derivatized-substance P and its metabolites by micellar electrokinetic chromatography.
Separation of naphthalene-2,3-dicarboxaldehyde-derivatized-substance P and its metabolites by micellar electrokinetic chromatography.
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通过胶束电动色谱法分离萘-2,3-二甲醛衍生物质 P 及其代谢物。
DOI：
10.1002/1522-2683(20000601)21:10
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Lunte,SM
中科院分区：
文献类型：
--
作者：
Freed,AL;Lunte,SM
This paper describes the development of analytical methodology for the separation of naphthalene‐2,3‐dicarboxaldehyde (NDA)‐derivatized substance P (CBI‐SP) and five lysine‐containing metabolites by micellar electrokinetic chromatography (MEKC). The effect of surfactant composition and organic modifiers on the separation was investigated. The final separation buffer consisted of 80 mMsodium cholate in 50 mMN‐tris (hydroxymethyl)methyl‐2‐aminoethanesulfonic acid (TES), pH 7. All six lysine‐containing peptides were separated under these conditions.
