A RAPID METHOD FOR PREPARING SYNAPTOSOMES - COMPARISON, WITH ALTERNATIVE PROCEDURES
A RAPID METHOD FOR PREPARING SYNAPTOSOMES - COMPARISON, WITH ALTERNATIVE PROCEDURES
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DOI：
10.1016/0006-8993(81)91086-6
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
DELAUNOY, JP
中科院分区：
文献类型：
--
作者：
DODD, PR;HARDY, JA;DELAUNOY, JP
本文介绍了一种从大鼠大脑皮层快速（1-1.5小时）制备神经末梢颗粒（突触体）的方法。突触体部分的特点是定量EM和酶分布的研究。该馏分显示出一定程度的富集突触结构，这是可比的标准（4-5小时）制备，并大大优于替代快速（2-2.5小时）的方法。在孵育，突触体获得的新程序积累了高浓度的K+的组织，并表现出高，线性速率的O2摄取。藜芦碱去极化引起呼吸速率和生理活性氨基酸的释放显著增加;谷氨酸、天冬氨酸和GABA，以及组织K+的显著减少。通过这些代谢和功能性能指标判断，新方法与替代方法相比毫不逊色。这种新的制备方法对人死后脑突触体的代谢研究具有一定的参考价值。
A method for the rapid (1-1.5 h) preparation of nerve ending particles (synaptosomes) from rat cerebral cortex is described. The synaptosome fraction was characterized by quantitative EM and enzyme distribution studies. The fraction showed a degree of enrichment in synaptic structures which was comparable to that of the standard (4-5 h) preparation, and substantially better than an alternative fast (2-2.5 h) method. On incubation, synaptosomes obtained by the new procedure accumulated a high tissue concentration of K+ and showed a high, linear rate of O2 uptake. Depolarization by veratrine caused a significant increase in the rate of respiration and in the release of the physiologically active amino acids; glutamate, aspartate and GABA, and a significant reduction in tissue K+. The new procedure compared favorably with alternative methods as judged by these indices of metabolic and functional performance. The new preparation method is of value in metabolic studies of synaptosomes prepared from human post-mortem brain.