Maid (GCIP) is involved in cell cycle control of hepatocytes
Maid (GCIP) is involved in cell cycle control of hepatocytes
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DOI：
10.1002/hep.21461
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发表时间：
2007-02-01
期刊：
影响因子：
13.5
通讯作者：
Riethmacher, Dieter
中科院分区：
文献类型：
--
作者：
Sonnenberg-Riethmacher, Eva;Wuestefeld, Torsten;Riethmacher, Dieter
通过靶向破坏小鼠体内的细胞周期蛋白D结合螺旋-环-螺旋蛋白Maid（GCIP）的功能进行了分析。我们表明，女仆功能是不需要正常的胚胎发育。然而，与野生型对照组相比，年龄较大的Maid缺陷型小鼠发生肝细胞癌。因此，我们研究了Maid在部分肝切除术（PH）后细胞周期进程中的作用。PH后缺乏Maid表达与G1/S期进展延迟相关，Maid缺陷小鼠中细胞周期蛋白A表达延迟和DNA复制延迟证明了这一点。然而，在稍后的时间点，肝脏质量恢复正常。结论：这些结果表明，Maid参与肝细胞的G1/S期进展，这在老年动物中与肝肿瘤的发展有关。
The function of Maid (GCIP), a cyclinD-binding helix-loop-helix protein, was analyzed by targeted disruption in mice. We show that Maid function is not required for normal embryonic development. However, older Maid-deficient mice-in contrast to wild-type controls-develop hepatocellular carcinomas. Therefore, we studied the role of Maid during cell cycle progression after partial hepatectomy (PH). Lack of Maid expression after PH was associated with a delay in G1/S-phase progression as evidenced by delayed cyclinA expression and DNA replication in Maid-deficient mice. However, at later time points liver mass was restored normally. Conclusion: These results indicate that Maid is involved in G1/S-phase progression of hepatocytes, which in older animals is associated with the development of liver tumors.