Adhesion of Human Osteoblast-like Cells (Saos-2 cells) on Micro/nanopatterned Structures Sputter-Coated with Titanium
Adhesion of Human Osteoblast-like Cells (Saos-2 cells) on Micro/nanopatterned Structures Sputter-Coated with Titanium
复制标题
人成骨细胞样细胞（Saos-2 细胞）在钛溅射微/纳米图案结构上的粘附
作者：
Kaga;Naoyuki Akasaka;Tsukasa Horiuchi;Rumi Yoshida;Yasuhiro Yokoyama;Atsuro
种植体的表面纹理采用了各种设计，以实现种植体上的骨结合，从而获得临床成功。在该体外研究中，通过纳米压印方法制备具有500 nm、1 μm和2 μm的沟槽和柱状形状的钛片。将成骨细胞样细胞（Saos-2细胞）以5000个细胞/cm 2的密度接种在Ti片上，并在含有10%FBS的DMEM中培养。通过计算已增殖的细胞数量并通过观察粘着斑蛋白形成以确定接种后24小时是否存在病灶接触来评价细胞活力。在槽和柱结构上，细胞在1小时时生长，并且在24小时时的细胞数量是作为对照的光滑表面上的大约两倍。在500-nm的凹槽和柱上的细胞数量高于其他图案。孵育24小时后，黏着斑蛋白形成已延伸到槽和柱结构的脊，作为细胞粘附的证据。黏着斑蛋白的增加与细胞分化相关。结果表明表面改性对钛片生物学效应的重要性以及表面改性在改善骨整合方面的潜在用途。
Various designs are used for surface textures of implants to achieve bone osseointegration on the implant for clinical success. In this in vitro study, Ti sheets with groove and pillar shapes of 500 nm, 1 μm, and 2 μm were made by a nanoimprinting method. Osteoblast-like cells (Saos-2 cells) were seeded on the Ti sheets at a density of 5000 cells/cm2 and cultured in DMEM containing 10% FBS. Cell viability was evaluated by calculating the number of cells that had proliferated and by observing vinculin formation to determine the presence of focal contacts 24 hours after seeding. On the groove and pillar structures, cells were growing at 1 hour and the number of cells at 24 hours was approximately twice that on smooth surfaces as a control. The number of cells on the 500-nm grooves and pillars was higher than that on the other patterns. After incubation for 24 hours, vinculin formation had extended to the ridges of the groove and pillar structures as an evidence of cell adhesion. The increase in vinculin was correlated with cell differentiation. The results indicate the importance of the biological effect of surface modification on Ti sheets and the potential use of surface modification to improve osseointegration.