The MAP kinase Mkc1p is activated under different stress conditions in Candida albicans
The MAP kinase Mkc1p is activated under different stress conditions in Candida albicans
复制标题
DOI：
10.1099/mic.0.28038-0
复制
发表时间：
2005-08-01
期刊：
影响因子：
2.8
通讯作者：
Pla, J
中科院分区：
文献类型：
--
作者：
Navarro-García, F;Eisman, B;Pla, J
白色念珠菌是一种条件致病菌，已适应在人体内作为其自然环境生活和生长。在这些条件下，这种真菌面临着许多挑战，包括可能破坏外部和内部结构的氧化，渗透和酶促过程。鉴于MAP激酶信号通路在C.白念珠菌，分析了模拟体内环境条件的试剂对p42-44 MAP激酶活化的影响。已经发现，Mkc 1 p在氧化应激、渗透压变化、细胞壁损伤和生长温度降低的存在下被磷酸化。这种磷酸化依赖于Pkc 1 p，表明这两种蛋白在C.白色念珠菌在某些刺激下，Mkc 1 p的磷酸化需要Hog 1 p（高渗透压甘油（HOG）途径的MAP激酶）的存在。这表明存在一个新的监管作用，至少在某些条件下，这些MAP激酶途径在酵母。
Candida albicans is an opportunistic pathogen that has adapted to live and grow in the human body as its natural environment. Under these conditions, this fungus faces numerous challenges, including oxidative, osmotic and enzymic processes that may damage external and internal structures. In view of the key role of MAP kinase signalling pathways in the physiology of C. albicans, the effect of agents mimicking in vivo environmental conditions on the activation of the p42-44 MAP kinases has been analysed. It has been found that Mkc1p is phosphorylated in the presence of oxidative stress, changes in osmotic pressure, cell wall damage and a decrease in the growth temperature. This phosphorylation is dependent on Pkc1p, indicating that both proteins operate in the same signalling pathway in C. albicans. Under some stimuli, the phosphorylation of Mkc1p required the presence of Hog1p, the MAP kinase of the high osmolarity glycerol (HOG) pathway. This suggests the existence of a new regulatory role, at least under some conditions, for these MAP kinase pathways in yeast.