COLORIMETRIC DETERMINATION OF FREE FATTY ACIDS IN BIOLOGICAL FLUIDS.
COLORIMETRIC DETERMINATION OF FREE FATTY ACIDS IN BIOLOGICAL FLUIDS.
复制标题
生物液体中游离脂肪酸的比色测定。
作者：
K. Itaya;M. Ui
继 Duncombe 介绍了用于测定溶解在氯仿中的游离脂肪酸 (FFA) 的比色微量方法之后，我们研究了从血液或其他生物液体中氯仿提取 FFA 的最佳条件。获得的提取程度和提取率与都乐提取混合物相似。磷脂不是通过所描述的程序提取的。只需 0.2 ml 全血，即可代替血清或血浆，作为后续比色测定中的 FFA 样品，该比色测定经过修改，使其更简单、更灵敏。
Following the introduction by Duncombe of a colorimetric micromethod for the determination of free fatty acids (FFA) dissolved in chloroform, we studied the optimum conditions for chloroform extraction of FFA from blood or other biological fluids. A degree and rate of extraction similar to those with Dole’s extraction mixture were obtained. Phospholipids are not extracted by the procedure described. As little as 0.2 ml of whole blood, instead of serum or plasma, can serve as a FFA sample in the subsequent colorimetric determination, which has been modified to render it simpler and more sensitive.