Wnt signalling mediated by Tbx2b regulates cell migration during formation of the neural plate
Wnt signalling mediated by Tbx2b regulates cell migration during formation of the neural plate
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DOI：
10.1242/dev.01933
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发表时间：
2005-08-01
期刊：
影响因子：
4.6
通讯作者：
Korzh, V
中科院分区：
文献类型：
--
作者：
Fong, SH;Emelyanov, A;Korzh, V
在原肠胚形成期间，对信号线索的最佳粘附性和接受性对于细胞通过协调的细胞运动获得新的位置和身份是必不可少的。已知T-box转录因子和Wnt信号通路在这些过程中发挥重要作用。斑马鱼tbx2b是TBX2家族的成员，先前已被证明是中线中胚层规格所需的。我们在这里表明，TBX2B转录本存在于中期原肠胚之前，其表达检测整装原位杂交。Tbx2b缺陷的分离的外胚层细胞具有改变的细胞表面性质，并且这些细胞中的钙粘蛋白水平较低。在通过细胞移植和单个卵裂球注射产生的嵌合体胚胎中，Tbx2b缺陷细胞在细胞自主方式的细胞运动中有缺陷，导致它们被排除在发育中的神经板之外。使用这种"排除"表型作为筛选，我们表明Tbx2b在Fz7信号传导的背景下起作用。在发育过程中，嵌合体中缺乏T-box蛋白的细胞被排除在其他T-box基因之外，这可能表明T-box蛋白的一般功能机制。
During gastrulation, optimal adhesion and receptivity to signalling cues are essential for cells to acquire new positions and identities via coordinated cell movements. T-box transcription factors and the Wnt signalling pathways are known to play important roles in these processes. Zebrafish tbx2b, a member of the TBX2 family, has previously been shown to be required for the specification of midline mesoderm. We show here that tbx2b transcripts are present during mid-gastrula before its expression is detected by whole-mount in situ hybridization. Isolated ectodermal cells deficient in Tbx2b have altered cell surface properties and the level of cadherins in these cells is lower. In chimaeric embryos generated by cell transplantation and single blastomere injections, Tbx2b-deficient cells are defective in cell movement in a cell-autonomous manner, resulting in their exclusion from the developing neural plate. Using this 'exclusion' phenotype as a screen, we show that Tbx2b acts within the context of Fz7 signalling. The exclusion of cells lacking T-box proteins in chimeras during development was demonstrated with other T-box genes and may indicate a general functional mechanism for T-box proteins.