RADIATION-INDUCED ASTROCYTIC AND MICROGLIAL RESPONSES IN MOUSE-BRAIN
RADIATION-INDUCED ASTROCYTIC AND MICROGLIAL RESPONSES IN MOUSE-BRAIN
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DOI：
10.1016/0167-8140(93)90174-7
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发表时间：
1993-10-01
影响因子：
5.7
通讯作者：
WITHERS, HR
中科院分区：
文献类型：
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作者：
CHIANG, CS;MCBRIDE, WH;WITHERS, HR
本研究旨在探讨星形胶质细胞和小胶质细胞对全脑照射的反应。胶质细胞酸性蛋白（GFAP）是星形胶质细胞的标志物，其水平通过ELISA在照射后不同时间点的照射脑中测量。GFAP水平在单次剂量20 - 45戈伊辐射后120 - 180天增加，但在较低剂量（2或8戈伊）后没有增加。通过Western印迹分析和免疫组织化学染色证实GFAP水平的增加，其显示GFAP阳性星形胶质细胞的数量增加，其染色强度也增加。与星形胶质细胞染色的增加相一致，Mac I抗原的小胶质细胞染色的数量和强度也增加。脑组织放射自显影[H-3]胸苷体内给药后，在同一时间段内标记细胞的数量增加。放射诱导的星形胶质细胞和小胶质细胞反应，脑照射后是反应性胶质细胞增生和炎症发生在潜伏期，直到晚期放射诱导的损伤的发作。这种胶质增生随着辐射剂量的增加而增加。胶质细胞增生可能参与修改postirradiation损伤的大脑进行了讨论。
The aim of this study was to investigate the responses of astrocytes and microglia to whole brain irradiation. Levels of glial fibrillary acidic protein (GFAP), which is a marker for astrocyte's, were measured by ELISA in irradiated brains taken at varying time points after irradiation. GFAP levels were increased between 120 and 180 days after single doses of 20-45 Gy radiation, but not after lower doses (2 or 8 Gy). The increases in GFAP levels were confirmed by Western blot analysis and immunohistochemical staining which showed that the number of GFAP-positive astrocytes was increased, as was their staining intensity. Coincidently with the increase in astrocyte staining, there was an increase in the number and the intensity of microglial cell staining for Mac I antigen. Autoradiography of brain tissue following in vivo administration of [H-3]thymidine showed an increased number of labelled cells during the same time period. The radiation-induced astrocytic and microglial responses that follows brain irradiation is indicative of reactive gliosis and inflammation occurring during the latent period up to the onset of late radiation-induced injury. This gliosis increases with radiation dose. The possibility that gliosis may participate in modifying postirradiation injury in the brain is discussed.