Comparative genomic sequence analysis of novel Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Kenya and three other previously sequenced Helicoverpa spp. NPVs
Comparative genomic sequence analysis of novel Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Kenya and three other previously sequenced Helicoverpa spp. NPVs
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DOI：
10.1007/s11262-009-0389-3
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发表时间：
2009-07
期刊：
影响因子：
1.6
通讯作者：
J. Ogembo;B. Caoili;M. Shikata;S. Chaeychomsri;Michihiro Kobayashi;M. Ikeda
中科院分区：
文献类型：
--
作者：
J. Ogembo;B. Caoili;M. Shikata;S. Chaeychomsri;Michihiro Kobayashi;M. Ikeda
从肯尼亚新克隆的一种新的幽门螺杆菌核型多角体病毒(HearNPV)-NNG1，其杀虫活性高于HearNPV-G4，而G4的杀虫活性也低于HearNPV-C1。在寻找可能与观察到的棉铃虫之间的毒力差异有关的基因和/或核苷酸序列时。对NNg1的全基因组进行了测序，并与以前测得的G4、C1型和棉铃虫核衣壳核型多角体病毒(HZ)基因组进行了比较。NNg1基因组全长132,425个碱基，共有143个开放阅读框架(ORF)，与G4、C1和HZ具有较高的氨基酸和核苷酸序列同源性。在G4和Hz3个NNg1 ORF5、ORF100和ORF124缺失，而NNg1和C1缺失G4/HzORF5的同源。另外三个ORF60(bro-b)、ORF119和ORF120以及一个直接重复序列(DR)是NNG1所特有的。相对于总的核苷酸序列同源性，NNg1hr与其他3个Helicoverpaspp中的同源物之间的序列同源性较低。NPV，尽管包含相同数量的hr，但位于基因组上基本上相同的位置。NNg1与其他三个Helicoverpaspp之间也存在差异。NPV在基因组上编码的溴基因的多样性。这些结果表明，一些假定的基因和核苷酸序列可能与观察到的不同毒力有关，但具体的基因和/或核苷酸序列尚未确定。
A newly clonedHelicoverpaarmigeranucleopolyhedrovirus (HearNPV) from Kenya, HearNPV-NNg1, has a higher insecticidal activity than HearNPV-G4, which also exhibits lower insecticidal activity than HearNPV-C1. In the search for genes and/or nucleotide sequences that might be involved in the observed virulence differences amongHelicoverpaspp. NPVs, the entire genome of NNg1 was sequenced and compared with previously sequenced genomes of G4, C1 andHelicoverpazeasingle-nucleocapsid NPV (Hz). The NNg1 genome was 132,425 bp in length, with a total of 143 putative open reading frames (ORFs), and shared high levels of overall amino acid and nucleotide sequence identities with G4, C1 and Hz. Three NNg1 ORFs, ORF5, ORF100 and ORF124, which were shared with C1, were absent in G4 and Hz, while NNg1 and C1 were missing a homologue of G4/Hz ORF5. Another three ORFs, ORF60 (bro-b), ORF119 and ORF120, and one direct repeat sequence (dr) were unique to NNg1. Relative to the overall nucleotide sequence identity, lower sequence identities were observed between NNg1hrs and the homologoushrs in the other threeHelicoverpaspp. NPVs, despite containing the same number ofhrs located at essentially the same positions on the genomes. Differences were also observed between NNg1 and each of the other threeHelicoverpaspp. NPVs in the diversity ofbrogenes encoded on the genomes. These results indicate several putative genes and nucleotide sequences that may be responsible for the virulence differences observed amongHelicoverpaspp., yet the specific genes and/or nucleotide sequences responsible have not been identified.