Longitudinal study of retinal degeneration in a rat using spectral domain optical coherence tomography.
Longitudinal study of retinal degeneration in a rat using spectral domain optical coherence tomography.
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使用谱域光学相干断层扫描对大鼠视网膜变性进行纵向研究。
DOI：
10.1364/oe.18.023435
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发表时间：
2010
期刊：
影响因子：
3.8
通讯作者：
Mirza Faisal Beg
中科院分区：
文献类型：
--
作者：
M. Sarunic;Azadeh Yazdanpanah;E. Gibson;Jing Xu;Yu;Sieun Lee;H. U. Saragovi;Mirza Faisal Beg
视觉科学家使用视网膜退行性疾病的啮齿动物模型来开发治疗方法，并了解疾病进展的机制。测量不同视网膜层厚度的变化为评估治疗效果提供了一个客观的指标。由于侵袭性组织学是终极的，只提供一个数据点，因此需要非侵入性成像手段来更好地研究进展，并减少研究中使用的动物数量。光学相干层析成像(OCT)已经成为人类眼科成像的主要成像方式，但直到最近才在啮齿动物视网膜成像方面引起了极大的关注。OCT提供微米级分辨率的视网膜横截面图像，可以测量视网膜层厚度。然而，为了对视觉科学家有用，需要测量视网膜表面的很大一部分。此外，由于视网膜厚度通常随距视神经头的距离而变化，因此以系统的方式对视网膜的所有区域进行采样至关重要。我们提出了一项OCT的纵向研究，以测量接受视神经切断的大鼠的视网膜变性，视神经切断是一种具有良好特征的视网膜快速变性形式。使用半自动分割算法对OCT在时间进程研究中获得的视网膜体积图像进行2D分割。然后，使用3D算法，对所有体积分割的视网膜表面的厚度测量进行量化。由此得到的视网膜厚度随时间的变化图代表了损伤期间视网膜表面的退化进程。这些计算工具补充了OCT视网膜体积采集，为视觉科学家研究啮齿动物提供了一个强大的工具。
Rodent models of retinal degenerative diseases are used by vision scientists to develop therapies and to understand mechanisms of disease progression. Measurement of changes to the thickness of the various retinal layers provides an objective metric to evaluate the performance of the therapy. Because invasive histology is terminal and provides only a single data point, non-invasive imaging modalities are required to better study progression, and to reduce the number of animals used in research. Optical Coherence Tomography (OCT) has emerged as a dominant imaging modality for human ophthalmic imaging, but has only recently gained significant attention for rodent retinal imaging. OCT provides cross section images of retina with micron-scale resolution which permits measurement of the retinal layer thickness. However, in order to be useful to vision scientists, a significant fraction of the retinal surface needs to be measured. In addition, because the retinal thickness normally varies as a function of distance from optic nerve head, it is critical to sample all regions of the retina in a systematic fashion. We present a longitudinal study of OCT to measure retinal degeneration in rats which have undergone optic nerve axotomy, a well characterized form of rapid retinal degeneration. Volumetric images of the retina acquired with OCT in a time course study were segmented in 2D using a semi-automatic segmentation algorithm. Then, using a 3D algorithm, thickness measurements were quantified across the surface of the retina for all volume segmentations. The resulting maps of the changes to retinal thickness over time represent the progression of degeneration across the surface of the retina during injury. The computational tools complement OCT retinal volumetric acquisition, resulting in a powerful tool for vision scientists working with rodents.
影响因子：
3.5
作者：
Zawadzki, Robert J.;Fuller, Alfred R.;Werner, John S.
通讯作者：
Werner, John S.