Decreases in pheromonal responses at the accessory olfactory bulb of mice with a deficiency of the alpha1B or beta3 subunits of voltage-dependent Ca2+-channels.
Decreases in pheromonal responses at the accessory olfactory bulb of mice with a deficiency of the alpha1B or beta3 subunits of voltage-dependent Ca2+-channels.
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DOI：
10.1248/bpb.29.437
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发表时间：
2006-03
影响因子：
2
通讯作者：
M. Murakami;Hitosi Matsui;Takeshi Shiraiwa;Takashi Suzuki;H. Sasano;E. Takahashi;M. Kashiwayanagi
中科院分区：
文献类型：
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作者：
M. Murakami;Hitosi Matsui;Takeshi Shiraiwa;Takashi Suzuki;H. Sasano;E. Takahashi;M. Kashiwayanagi
信息素影响性腺功能和性行为。有关信息素的信息由犁鼻器 (VNO) 接收并传输到辅助嗅球 (AOB)。我们使用α1B缺陷小鼠和β3缺陷小鼠研究了N（神经元）型电压依赖性Ca2+通道的α1B和β3亚基在辅助嗅觉（犁鼻）系统神经转导中的生理作用。 RT-PCR 研究表明，小鼠 VNO 中存在电压依赖性 Ca2+ 通道的 beta1、beta2、beta3、beta4、alpha1A、alpha1B 和 alpha1C 亚基。免疫组织化学研究表明，电压依赖性 Ca2+ 通道的 α1A、α1B 和 α1C 亚基存在于小鼠 VNO 的感觉神经元和支持细胞中。将 VNO 暴露于雄性小鼠分泌的尿液样本中，α1B 缺陷雌性小鼠 AOB 的球周 (PG) 细胞中的 Fos 免疫反应性低于野生小鼠。暴露于雌性尿液样本后，α1B缺陷雄性小鼠肾小球周围细胞层的Fos免疫反应（Fos-ir）细胞密度低于野生小鼠。将缺乏β3的雌性小鼠的VNO暴露于雄性尿液样本中也会诱导AOB的肾小球周围细胞层中的Fos-ir细胞减少。这些数据表明 N 型电压依赖性 Ca2+ 通道的 alpha1B 和 beta3 亚基对于信息素信号转导系统的重要性。
Pheromones affect gonadal functions and sexual behaviors. Information in regard to pheromones is received by the vomeronasal organ (VNO) and transmitted to the accessory olfactory bulb (AOB). We investigated the physiological role of the alpha1B and beta3 subunits of the N (neuronal)-type voltage-dependent Ca2+ channel in the neurotransduction in the accessory olfactory (vomeronasal) system using alpha1B-deficient mice and beta3-deficient mice. RT-PCR studies showed the existence of beta1, beta2, beta3, beta4, alpha1A, alpha1B, and alpha1C subunits of voltage-dependent Ca2+ channels in the mouse VNO. Immunohistochemical studies showed that the alpha1A, alpha1B, and alpha1C subunits of voltage-dependent Ca2+ channels exist in the sensory neurons and supporting cells of the mouse VNO. Exposure of the VNO to urine samples excreted from male mice induced lower Fos-immunoreactivity in the periglomerular (PG) cells of the AOBs in alpha1B-deficient female mice than in those of wild mice. The density of Fos-immunoreactive (Fos-ir) cells after exposure to female urine samples at the periglomerular cell layer of alpha1B-deficient male mice was lower than that of wild mice. Exposure of the VNO of beta3-deficient female mice to male urine samples also induced low Fos-ir cells in the periglomerular cell layer of the AOB. These data suggest the importance of the alpha1B and beta3 subunits of the N-type voltage-dependent Ca2+ channel for the pheromone signal transduction system.