Membrane order in the plasma membrane and endocytic recycling compartment.
Membrane order in the plasma membrane and endocytic recycling compartment.
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DOI：
10.1371/journal.pone.0188041
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Maxfield FR
中科院分区：
文献类型：
--
作者：
Iaea DB;Maxfield FR
膜的胆固醇含量在组织膜进行信号转导和蛋白质运输以及调节膜的生物物理特性方面起着重要作用。虽然模型膜或分离膜的性质已经被广泛研究，但对活细胞内膜的评价很少。在这里，我们使用尼罗河红探针NR12S和比例活细胞成像来分析质膜和内吞循环室的膜顺序。我们发现，经过短暂的孵育以允许内吞作用后，NR12S分布在质膜和内吞循环室之间。NR12S报告说，内吞循环室比质膜更有序。我们还发现，改变细胞胆固醇水平对质膜和内吞循环室的影响是不同的。当细胞胆固醇含量增加或减少20%时，质膜的膜序发生显著改变，但内吞循环室不发生改变。这些结果表明，细胞内胆固醇水平的变化对不同细胞器内的膜序有不同的改变。
The cholesterol content of membranes plays an important role in organizing membranes for signal transduction and protein trafficking as well as in modulating the biophysical properties of membranes. While the properties of model or isolated membranes have been extensively studied, there has been little evaluation of internal membranes in living cells. Here, we use a Nile Red based probe, NR12S, and ratiometric live cell imaging, to analyze the membrane order of the plasma membrane and endocytic recycling compartment. We find that after a brief incubation to allow endocytosis, NR12S is distributed between the plasma membrane and the endocytic recycling compartment. The NR12S reports that the endocytic recycling compartment is more highly ordered than the plasma membrane. We also find that the plasma membrane and the endocytic recycling compartment are differentially affected by altering cellular cholesterol levels. The membrane order of the plasma membrane, but not the endocytic recycling compartment, is altered significantly when cellular cholesterol content is increased or decreased by 20%. These results demonstrate that changes in cellular cholesterol differentially alter membrane order within different organelles.
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