Passive stiffness of Drosophila IFM myofibrils: a novel, high accuracy measurement method.
Passive stiffness of Drosophila IFM myofibrils: a novel, high accuracy measurement method.
复制标题
果蝇 IFM 肌原纤维的被动刚度：一种新颖的高精度测量方法。
DOI：
10.1007/s10974-004-0684-5
复制
发表时间：
2004
影响因子：
2.7
通讯作者：
Pollack,GeraldH
中科院分区：
文献类型：
--
作者：
Hao,Yudong;Bernstein,SanfordI;Pollack,GeraldH
作为保留完整收缩装置的最小肌细胞亚结构，单个肌原纤维被认为是肌肉力学的最佳标本，尽管其小尺寸也带来了一些技术困难。果蝇直接飞行肌（IFM）的肌原纤维是一种很难研究的肌原纤维，因为它们的被动刚度很大，很难处理，而且很难拉伸，不能产生足够的伸长来精确测量肌节长度的变化。在这项研究中，我们设计了一种新的方法，精确测量单松弛肌原纤维的刚度使用微制造的杠杆和相差显微镜。一个特殊的实验协议，以尽量减少错误，并使用一些数据分析策略，以识别和排除虚假数据。从果蝇IFM肌原纤维中获得了非常一致的结果。这种新的，高精度的方法是潜在的一个有效的工具，用于检测肌肉突变体的小被动刚度变化。
As the smallest muscle-cell substructure that retains the intact contractile apparatus, the single myofibril is considered the optimal specimen for muscle mechanics, although its small size also poses some technical difficulties. Myofibrils fromDrosophilaindirect flight muscle (IFM) are particularly difficult to study because their high passive stiffness makes them hard to handle, and too resistant to stretch to produce enough elongation for the accurate measurement of sarcomere length change. In this study, we devised a novel method for accurate stiffness measurement of single relaxed myofibrils using microfabricated cantilevers and phase contrast microscopy. A special experimental protocol was developed to minimize errors, and some data analysis strategies were used to identify and exclude spurious data. Remarkably consistent results were obtained fromDrosophilaIFM myofibrils. This novel, high accuracy method is potentially an effective tool for detecting small passive stiffness change in muscle mutants.