The expression of PEBP4 protein in lung squamous cell carcinoma
The expression of PEBP4 protein in lung squamous cell carcinoma
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DOI：
10.1007/s13277-011-0230-1
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发表时间：
2011-09
期刊：
影响因子：
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通讯作者：
Guiping Yu;Guo-qiang Chen;Song Wu;Kai Shen;Yong Ji
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文献类型：
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作者：
Guiping Yu;Guo-qiang Chen;Song Wu;Kai Shen;Yong Ji
磷脂酰乙醇胺结合蛋白4(PEBP4)是PEBP家族的成员。它不仅在抑制MAPK信号通路中发挥作用，而且还参与抑制促进AKT活化的JNK通路。最近的研究也表明，PEBP4的过度表达与多种肿瘤的发生、侵袭和转移有关。本研究旨在探讨PEBP4蛋白在肺鳞癌组织中的表达与肺鳞癌临床病理的关系。采用免疫组织化学方法检测61例肺鳞癌组织及癌旁正常组织中PEBP4的表达。免疫印迹法检测肺鳞癌组织与癌旁正常组织中PEBP4蛋白表达的变化。分析PEBP4表达与肺鳞癌发生、发展及临床病理的关系。在61例患者中，4例PEBP-4阴性(−；6.6%)，57例阳性(+到+；93.4%)。PEBP4表达阳性者中，弱阳性7例(+；11.5%)，阳性21例(++；34.4%)，强阳性29例(+；47.5%)。PEBP4蛋白在肺鳞癌组织中的表达高于癌旁正常肺组织(p<； )。在PEBP4阳性患者中，有淋巴结转移者的PEBP4蛋白表达显著高于无转移者(p<； )。早期(I和II期)患者的PEBP4表达显著低于晚期(III和IV期)患者(p<； 0.05)。在分化较低的肺鳞癌中，PEBP4蛋白的表达较高(p<； )；然而，这与患者的性别、年龄或肿瘤大小无关(p>； 0.05)。PEBP4蛋白过表达与肺鳞癌的发生、侵袭和转移有关。
The phosphatidylethanolamine-binding protein 4 (PEBP4) is a member of the PEBP family. It not only plays a role in the inhibition of the MAPK signaling pathway but also is involved in the inhibition of the JNK pathway that promotes the activation of AKT. Recent research has also shown that overexpression of PEBP4 was related to the development, invasion, and metastasis of a variety of tumors. This study aimed to investigate the correlation between PEBP4 protein expression in lung squamous cell carcinoma tissue and the clinical pathology of lung squamous cell carcinoma. Immunohistochemistry was used to detect PEBP4 expression in lung squamous cell carcinoma tissue and adjacent normal tissue from 61 patients. Western blotting was used to detect changes in the expression of PEBP4 protein between lung squamous cell carcinoma tissue and adjacent normal tissues. The correlation of PEBP4 expression and the occurrence, development, and clinical pathology of lung squamous cell carcinoma was analyzed. Of 61 patients, four patients were PEBP4 negative (−; 6.6%) and 57 patients were positive (+ to +++; 93.4%). Of those positive for PEBP4 expression, 7 patients were weakly positive (+; 11.5%), 21 patients were positive (++; 34.4%), and 29 patients were strongly positive (+++; 47.5%). PEBP4 protein was more highly expressed in lung squamous cell carcinoma tissue than in the adjacent normal lung tissue (p< 0.05). In PEBP4-positive patients, PEBP4 protein expression was significantly greater in those with lymph node metastases than in those without (p< 0.05). PEBP4 expression was significantly lower in patients at early (I and II) stages than in patients at advanced (III and IV) stages (p< 0.05). In less differentiated lung squamous cell carcinomas, PEBP4 protein expression was greater (p< 0.05); however, this was unrelated to the gender, age, or tumor size of the patient (p> 0.05). PEBP4 protein overexpression was associated with the occurrence, invasion, and metastasis of lung squamous cell carcinoma.