Comparative Tolerances of Various Beauveria bassiana Isolates to UV-B Irradiation with a Description of a Modeling Method to Assess Lethal Dose
Comparative Tolerances of Various Beauveria bassiana Isolates to UV-B Irradiation with a Description of a Modeling Method to Assess Lethal Dose
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DOI：
10.1007/s11046-009-9207-7
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发表时间：
2009-09-01
期刊：
影响因子：
5.5
通讯作者：
Feng, Ming-Guang
中科院分区：
文献类型：
--
作者：
Huang, Bao-Fu;Feng, Ming-Guang
通过多剂量生物测定定量评估了来自全球宿主昆虫的 20 种白僵菌分离株对 UV-B 辐射的耐受性。将涂在载玻片上的分离物的分生孢子悬浮液暴露于0.1-1.6 J cm(-2) (D)的梯度UV-B剂量，该剂量在2.0-2.61 mW cm(-2)的加权312 nm波长照射0.75至10.17分钟时产生。然后将经照射的分生孢子在25A摄氏度和饱和湿度下孵育24小时。将各剂量下的发芽率与空白对照中的发芽率的比率定义为存活指数(I(s))。对于所有分离株，I (s) - D 观察结果与生存模型 I (s) = 1/[1 + exp(a + bD)] （0.94 千分之一货币符号 r (2) 千分之一货币符号 0.99）非常吻合，产生了广泛的致死剂量 0.154-0.928、0.240-1.139 和 0.383-1.493 J cm(-2) 分别损失 50%、75% 和 95% 的活力。这些远低于夏季晴天测量的太阳 UV-B 剂量 2.439 J cm(-2)。不同菌株对 UV-B 耐受性的巨大差异表明，有必要为夏季昆虫控制制剂选择耐受 UV-B 的候选制剂。开发了高效生物测定方法来准确测量真菌生防剂作为致死剂量的 UV-B 耐受性。
The tolerances of 20 Beauveria bassiana isolates derived from host insects worldwide to UV-B irradiation were assessed quantitatively in multi-dose bioassays. Conidial suspensions of the isolates smeared on glass slides were exposed to the gradient UV-B doses of 0.1-1.6 J cm(-2) (D), which generated from 0.75 to 10.17 min irradiation of weighted 312-nm wavelength at 2.0-2.61 mW cm(-2). Irradiated conidia were then incubated for 24 h at 25A degrees C under saturated humidity. The ratio of germination at each dose over that in the blank control was defined as survival index (I (s)). For all isolates, the I (s) - D observations fit well with the survival model I (s) = 1/[1 + exp(a + bD)] (0.94 a parts per thousand currency sign r (2) a parts per thousand currency sign 0.99) generated widely spanned lethal doses of 0.154-0.928, 0.240-1.139, and 0.383-1.493 J cm(-2) for their losses of 50%, 75%, and 95% viabilities, respectively. These were far below the solar UV-B dose of 2.439 J cm(-2) measured in a sunny day during the summer. The large variation of UV-B tolerance among the isolates indicates a necessity to select UV-tolerant candidates for formulations applied to insect control during summer. The highly efficient bioassay method was developed to measure accurately the UV-B tolerances of fungal biocontrol agents as lethal doses.