Metabolic clearance of biologically active luteinizing hormone in man.
Metabolic clearance of biologically active luteinizing hormone in man.
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人体生物活性黄体生成激素的代谢清除。
DOI：
10.1172/jci112411
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Dufau,ML
中科院分区：
文献类型：
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作者：
Veldhuis,JD;Fraioli,F;Rogol,AD;Dufau,ML
采用大鼠间质细胞睾酮（RICT）生物测定法，研究了6例促性腺功能低下男性在单次注射高纯度人黄体生成素（LH）和连续注射3次分级剂量黄体生成素后血浆代谢清除率的变化。LH丸消失曲线提供的代谢清除率（MCR）估计为生物活性LH为24.1 +/- 4.7 (+/- SD) ml/min，而免疫活性LH为56.2 +/- 12 ml/min （P = 0.03）。连续输注生理剂量LH时，生物活性LH的MCR低于免疫活性LH；例如，生物活性LH和免疫活性LH的平均稳态mcr分别为26.1 +/- 3.1和34.2 +/- 3.2 ml/min （P = 0.02）。此外，逐步剂量输注方案允许我们证明，增加剂量的纯人LH导致生物活性和免疫活性LH的表观mcr进行性和平行降低。根据免疫活性和生物活性LH的生理血浆浓度计算的各自稳态mcr，我们估计正常男性生物活性激素的平均内源性产生率为1937 IU/24 h，免疫活性LH的平均内源性产生率为589 IU/24 h。这些结果表明，先前仅从免疫测定数据估计的黄体生成率明显低估了人体分泌的生物活性激素的数量。
The plasma metabolic clearance of biologically active luteinizing hormone (bioactive LH) was studied using the rat interstitial cell testosterone (RICT) bioassay in six hypogonadotropic men after single bolus injection of highly purified human LH and during continuous steady-state infusions of three graded doses of LH. The LH bolus disappearance curves provided estimates of metabolic clearance rates (MCR) of 24.1 +/- 4.7 (+/- SD) ml/min for bioactive LH vs. 56.2 +/- 12 ml/min for immunoactive LH in the same men (P = 0.03). A lower MCR of bioactive LH compared with immunoactive LH was also observed during continuous infusions of physiological doses of LH; for example, the mean steady-state MCRs for bioactive and immunoactive LH were, respectively, 26.1 +/- 3.1 and 34.2 +/- 3.2 ml/min (P = 0.02). Moreover, the stepped-dose infusion regimens permitted us to demonstrate that increasing doses of pure human LH resulted in progressive and parallel decreases in the apparent MCRs of both bioactive and immunoactive LH. Based on the respective steady-state MCRs calculated at physiological plasma concentrations of immunoactive and bioactive LH, we estimate a mean endogenous production rate for bioactive hormone of 1,937 IU/24 h, and for immunoactive LH of 589 IU/24 h in normal men. These results indicate that previous estimates of LH production rates from immunoassay data alone markedly underestimate the quantity of biologically active hormone secreted in man.