Current methods in investigating the development of the female reproductive system.
Current methods in investigating the development of the female reproductive system.
复制标题
目前研究女性生殖系统发育的方法。
DOI：
10.1007/978-1-60327-009-0_8
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Keating,AileenF
中科院分区：
文献类型：
--
作者：
Devine,PatrickJ;Hoyer,PatriciaB;Keating,AileenF
女性生殖系统对于卵母细胞的发育和受精、胚胎的植入和发育以及胎儿的生长和分娩都是重要的。它还会产生蛋白质和类固醇激素，帮助维持女性的健康。虽然女性表型是泌尿生殖系统发育的默认途径，但在发育过程中和发育后，许多过程可能会中断，这可能是由于发育问题造成的。不适当的发育可能是结构畸形、亚或不育、激素异常、子宫内膜异位症、癌症或其他有害后果的根本原因。我们的研究项目检查女性生殖系统的正常生理和功能，以及它如何因病理或环境/治疗暴露而受损，重点关注卵巢、卵巢卵泡和卵巢激素。本章将描述体外器官培养系统的详细方案和分析卵泡形成、卵泡发育和卵巢生理变化的方法。这些方法也可以应用于女性生殖的其他方面的研究。
The female reproductive system is important as the site for development and fertilization of an oocyte, for implantation and development of an embryo, and for growth and delivery of the fetus. It also produces protein and steroid hormones that help maintain a female’s health. Although the female phenotype is the default pathway for the development of the urogenital system, many processes can become disrupted during and after development which may originate from developmental problems. Improper development can be the underlying cause of structural malformations, sub- or infertility, hormonal abnormalities, endometriosis, carcinogenesis, or other detrimental outcomes. Our research programs examine the normal physiology and function of the female reproductive system and how it can become damaged due to pathologies or environmental/therapeutic exposures, with a focus on the ovary, ovarian follicles, and ovarian hormones. This chapter will describe detailed protocols of an in vitro organ culture system and methods to analyze changes in follicle formation, follicle development, and ovarian physiology. These methods can also be applied to the study of other aspects of female reproduction.