Vaccine and oncogenic strains of gallid herpesvirus 2 contain specific subtype variations in the 5' region of the latency-associated transcript that evolve in vitro and in vivo.
Vaccine and oncogenic strains of gallid herpesvirus 2 contain specific subtype variations in the 5' region of the latency-associated transcript that evolve in vitro and in vivo.
复制标题
鸡疱疹病毒 2 型的疫苗和致癌菌株在体外和体内进化的潜伏相关转录物的 5 区域含有特定的亚型变异。
DOI：
10.1007/s00705-014-2248-3
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发表时间：
2015
影响因子：
2.7
通讯作者：
Labaille J
中科院分区：
文献类型：
--
作者：
Labaille J
Gallid herpesvirus 2 (GaHV-2) is the alphaherpesvirus responsible for Marek’s disease (MD), a T-cell lymphoma of chickens. The virulence of the GaHV-2 field strain is steadily increasing, but MD is still controlled by the CVI988/Rispens vaccine. We tried to determine distinguishing traits of the CVI988/Rispens vaccine by focusing on the 5′ end region of the latency-associated transcript (5′LAT). It includes a variable number of 60-bp tandem repeats depending on the GaHV-2 strain. By analyzing six batches of vaccine, we showed that CVI988/Rispens consisted of a population of 5′LATmolecular subtypes, all with deletions and lacking 60-bp tandem repeat motifs, with two major subtypes that probably constitute CVI988/Rispens markers. Serial passages in cell culture led to a substantial change in the frequency of CVI988/Rispens 5′LATsubtypes, with non-deleted subtypes harboring up to four 60-bp repeats emerging during the last few passages. Dynamic changes in the distribution of 5′LAT-deleted subtypes were also detected after infection of chickens. By contrast, the 5′LATregion of the oncogenic clonal RB-1B strain, which was investigated at every step from the isolation of the clonal bacmid RB-1B DNA to the isolation of the ovarian lymphoma cell line, consisted of non-deleted 5′LATsubtypes harboring at least two 60-bp repeats. Thus, vaccine and oncogenic GaHV-2 strains consist of specific populations of viral genomes that are constantly evolvingin vivoandin vitroand providing potential markers for epidemiological surveys.
