A simple and reliable method for the determination and localization of chitin in abalone nacre
A simple and reliable method for the determination and localization of chitin in abalone nacre
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DOI：
10.1021/cm001217v
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发表时间：
2002-08-01
影响因子：
8.6
通讯作者：
Fritz, M
中科院分区：
文献类型：
--
作者：
Weiss, IM;Renner, C;Fritz, M
我们收集的测试反应，可以在一个简单的和可重复的方式应用于鲍鱼有机基质的化学组成。对鲍鱼珍珠层的层间有机基质进行了化学和生化测试反应，以研究多糖的含量和性质。通过光学显微镜平行检查多糖基质的制备。多糖核心被蜂窝状结构覆盖，其可以在释放疏水性氨基酸脯氨酸的情况下被蛋白酶枯草杆菌蛋白酶完全去除。蜂窝状图案的大小和形状与文石片的逆矩阵完全相同，文石片是众所周知的鲍鱼壳的珍珠层。本实验以一种简单明了的方法证明了鲍鱼中的多糖为甲壳素。此外，鲍鱼珍珠层的层间有机基质的H-1和C-13 NMR分析证实，它在很大程度上由多糖壳聚糖或其部分/完全N-乙酰化的衍生物几丁质（β-（1->4）-2-乙酰氨基-2-脱氧-D-葡萄糖或N-乙酰基-D-葡萄糖胺）组成。
We collected test reactions which can be applied in an easy and reproducible way to the chemical composition of the abalone organic matrix. Several chemical and biochemical test reactions were applied to the interlamellar organic matrix of abalone nacre to study the content and nature of polysaccharides. The preparation of the polysaccharide matrix was examined in parallel by light microscopy. The polysaccharide core is covered by a honeycomb-like structure, which can be completely removed by the protease subtilisin under release of the hydrophobic amino acid proline. The honeycomb pattern is in its size and its shape exactly the inverse matrix of the aragonite tablets, which are well-known to build up the nacreous layer of abalone shells. With this protocol we proved and verified in a straightforward and simple way the polysaccharides in abalone to be chitin. In addition, H-1 and C-13 NMR analysis of the interlamellar organic matrix of abalone nacre confirmed that it consists to a high extent of the polysaccharide chitosan or its partially/completely N-acetylated derivative chitin (beta-(1-->4)-2-acetamido-2-deOXY-D-glucose or N-acetyl-D-glucosamine).