Simple and effective 3D recognition of domoic acid using a molecularly imprinted polymer
Simple and effective 3D recognition of domoic acid using a molecularly imprinted polymer
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DOI：
10.1021/ja0741426
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发表时间：
2007-11-07
影响因子：
15
通讯作者：
Kaya, Kunimitsu
中科院分区：
文献类型：
--
作者：
Nemoto, Koji;Kubo, Takuya;Kaya, Kunimitsu
我们发展了一种简单有效的分子印迹技术，可以对具有柔性结构的目标化合物进行分子印迹。软骨藻酸（DA），一种健忘性贝类毒素，被用作目标化合物，而许多酸性化合物（单，二，三羧酸）被用作分子印迹聚合物（MIPs）的模板分子。使用液相色谱法的选择性识别能力的评价表明，最高的选择性识别能力时，发现戊烷-1，3，5-三羧酸（1，3，5-PeTA）作为模板。DA结构的计算机模拟研究表明，所观察到的选择性识别依赖于在识别位点的MIP，以及DA和1，3，5-PeTA中的COOH基团之间的空间距离的DA的结构变化。利用1，3，5-PeTA-MIP，我们可以很容易地通过固相萃取法从贻贝提取物中纯化DA。
We have developed a simple and effective molecular imprinting technique to target compounds with flexible structure. Domoic acid (DA), an amnesic shellfish poison, was used as the target compound while many acidic compounds (mono-, di-, and tricarboxylic acids) were used as template molecules for molecularly imprinted polymers (MIPs). Evaluation of selective recognition abilities using liquid chromatography revealed that the highest selective recognition ability for DA was found when pentane-1,3,5-tricarboxylic acid (1,3,5-PeTA) was used as the template. Computer modeling studies of the DA structure suggested that the observed selective recognition depended on the structural changes in DA at the recognition site of the MIPs as well as spatial distance between the COOH groups in DA and 1,3,5-PeTA. Using the 1,3,5-PeTA-MIP, we could easily purify DA from blue mussel extracts by solid-phase extraction.