Engineering Synthetic Myosin Filaments Using DNA Nanotubes.
Engineering Synthetic Myosin Filaments Using DNA Nanotubes.
复制标题
使用 DNA 纳米管工程合成肌球蛋白丝。
DOI：
10.1007/978-1-4939-8556-2_5
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Sivaramakrishnan,Sivaraj
中科院分区：
文献类型：
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作者：
Sommese,RuthF;Sivaramakrishnan,Sivaraj
在整个细胞中，马达蛋白协同工作以驱动许多分子过程和功能。例如，肌球蛋白马达的集合体共同运输囊泡和细胞器，维持膜稳态，并驱动肌肉收缩。在群体中研究这些运动变得越来越重要，因为研究表明，群体行为的出现与个体运动行为不同。在过去十年中使用的一种强大的技术是DNA纳米技术，它提供了对模式化马达蛋白的间隔和组织的精确控制。然而，直到最近，大多数结合DNA纳米结构和分子马达的研究都局限于离散的DNA结构，马达蛋白的附着点有限。在这一章中，我们描述了一种新的方法，使用DNA纳米管制造合成电机丝。我们目前的方法制备肌球蛋白VI标记的纳米管和测试这些纳米管使用一般的体外运动设置。总的来说，这些纳米管可以很容易地用于研究其他大型分子马达，如肌肉肌球蛋白或纤毛动力蛋白，这两种蛋白质在大型马达中工作，以驱动关键的细胞功能。
Throughout the cell, motor proteins work together to drive numerous molecular processes and functions. For example, ensembles of myosin motors collectively transport vesicles and organelles, maintain membrane homeostasis, and drive muscle contraction. Studying these motors in groups has become increasingly important with work demonstrating the emergence of ensemble behavior distinct from individual motor behavior. One powerful technique that has been used in the last decade is DNA nanotechnology, which provides precise control over spacing and organization of patterned motor proteins. Until recently, however, most studies combining DNA nanostructures and molecular motors have been confined to discrete DNA structures with limited attachment points for motor proteins. In this chapter, we describe a new approach for making synthetic motor filaments using DNA nanotubes. We present methods for preparing myosin VI-labeled nanotubes and testing these nanotubes using a general in vitro motility setup. Overall, these nanotubes can easily be used to study other large ensembles of molecular motors, such as muscle myosin or ciliary dynein, both proteins that work in large motor ensembles to drive key cellular functions.