Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit
Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit
复制标题
氮亏缺下转基因棉花苏云金芽孢杆菌杀虫蛋白含量降低
DOI：
10.1016/s2095-3119(20)63190-2
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发表时间：
2021-01-01
影响因子：
4.8
通讯作者：
Chen De-hua
中科院分区：
文献类型：
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作者：
Chen, Yuan;Liu Zhen-yu;Chen De-hua
为明确氮亏缺对方形Bt杀虫蛋白含量的影响，在2015-2016年棉花生育季，对常规品种思康1号（SK-1）和杂交品种思康3号（SK-3）施加不同的施氮量（0、75、150、225和300 kg ha(-1)）。不同施氮量下，当施氮量由常规施氮量（300 kg ha(-1)）降至0 kg ha(-1)时，单株平方数、平方体积和平方干重均减少。且方形Bt蛋白含量相应降低。氮代谢分析表明，缺氮条件下可溶性蛋白含量、GPT和GOT活性降低，游离氨基酸、肽酶和蛋白酶活性升高。相关分析表明，氮缺乏下Bt蛋白含量的降低与氮代谢的改变有关。综上所述，缺氮条件下，方形发育和方形Bt毒素含量均下降，表明在适当施氮量下促进方形发育也能提高现蕾期的抗虫性。
To clarify the effect of the N deficit on the amount of square Bt insecticidal protein, different N application rates (0, 75, 150, 225, and 300 kg ha(-1)) were imposed on the conventional cultivar Sikang 1 (SK-1) and hybrid cultivar Sikang 3 (SK-3) during 2015-2016 cotton growth seasons. Under different N application rates, the square number per plant, square volume and square dry weight reduced when the N rates decreased from conventional rate (300 kg ha(-1)) to 0 kg ha(-1). And the square Bt protein content decreased accordingly. The analysis of N metabolism showed that soluble protein content, GPT and GOT activities decreased, free amino acid, peptidase and protease activities increased under N deficit. Correlation analysis indicated that the reduced Bt protein content under N deficit was related to altered N metabolism. In conclusion, square development and the amount of square Bt toxin both decreased under N deficit, indicating that promoting the square development under appropriate N application rate would also promote the insect resistance during squaring stage.