Polyphenolics content and biological activity of Plectranthus amboinicus (Lour.) spreng growing in Egypt (Lamiaceae).
Polyphenolics content and biological activity of Plectranthus amboinicus (Lour.) spreng growing in Egypt (Lamiaceae).
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DOI：
10.5530/pj.2012.32.9
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Sleem, A. A.
中科院分区：
文献类型：
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作者：
El-Hawary, S. S.;El-Sofany, R. H.;Sleem, A. A.
背景资料：香茶菜属植物的挥发油、萜类化合物（主要是二萜）和多酚类化合物（包括黄酮类和酚酸类化合物）已被分离。香茶Plectanthus amboinicus（Lour.）对国外生长的春黄菊进行了植物化学研究，分离出几种黄酮类化合物，并具有抗氧化、利尿、抗炎、细胞毒性和抗菌活性。材料与方法：本研究以香茶Plectanthus amboinicus（Lour.）对埃及产的春叶进行了分级分离，并通过硅胶和葡聚糖凝胶色谱分离其酚类成分。分离得到的化合物经UV、1HNMR和13 CNMR鉴定。测定了海南香茶（Plectanthus amboinicus（Lour.）分别使用Folin-Ciocalteau和Folin-Denis试剂测定Spreng。茎和根的酚类化合物使用UPLC-MS分析鉴定。对该植物的叶、茎和根进行了抗氧化、抗炎、镇痛、利尿、细胞毒性和抗菌活性测试。结果如下：分离得到的化合物经鉴定为3-甲氧基芫荽素、克里斯马里汀、对香豆酸、咖啡酸、紫杉叶素、迷迭香酸、芹菜素和5-O-甲基-木樨草素。茎显示出最高浓度的总多酚，其次是叶，然后是根（9.6，8.4和5.4毫克/克没食子酸当量，分别），而根记录最高的单宁含量，其次是叶，然后茎（126，90和81克/克单宁酸当量，分别）。UPLC-MS分析表明，咖啡酸，迷迭香酸，香豆酸和chrysoeriol的茎和根中的存在下，而木犀草素，槲皮素和eriodyctiol只在茎中检测到。这三种器官的不同提取物具有抗氧化、抗炎、镇痛、利尿、细胞毒性和抗菌活性，效力各不相同。
Background: Volatile oil, terpenoids, mainly diterpene and polyphenolic compounds including flavonoids and phenolic acids were previously isolated from different Plectranthus species. Plectranthus amboinicus (Lour.) Spreng growing abroad was subjected to phytochemical study resulted in isolation of several flavonoids, also the plant exhibited antioxidant, diuretic, anti-inflammatory, cytotoxic and antimicrobial activities. Materials and Methods: In this study ethyl acetate fraction of Plectranthus amboinicus (Lour.) Spreng leaves growing in Egypt was fractionated and chromatographed on silica gel and sephadex to isolate its phenolic constituents. The isolated compounds were identified using UV, 1HNMR and 13CNMR. Total phenolics and tannins content of the leaves, stems and roots of Plectranthus amboinicus (Lour.) Spreng were determined using Folin-Ciocalteau and Folin-Denis reagents, respectively. Phenolic compounds of the stems and roots were identified using UPLC-MS analysis. Leaves, stems and roots of this plant were tested for antioxidant, anti-inflammatory, analgesic, diuretic, cytotoxic and antimicrobial activities. Results: The isolated compounds were identified as 3-methoxy genkwanin, crisimaritin, p-coumaric acid, caffeic acid, taxifolin, rosmarinic acid, apigenin and 5-O-methyl-luteolin. The stems showed the highest concentration of the total polyphenolics followed by the leaves then the roots (9.6, 8.4 and 5.4 mg/g of gallic acid equivalents, respectively), while the roots recorded the highest tannins content followed by the leaves then the stems (126, 90 and 81 g/g of tannic acid equivalents, respectively). UPLC-MS analysis revealed the presence of caffeic acid, rosmarinic acid, coumaric acid and chrysoeriol in the stems and roots, while luteolin, quercitin and eriodyctiol were detected only in the stems. The different extracts of the three organs exhibited antioxidant, anti-inflammatory, analgesic, diuretic, cytotoxic and antimicrobial activities with variable potency.