Interactions Between Plant Viral Nanoparticles (VNPs) and Blood Plasma Proteins, and Their Impact on the VNP In Vivo Fates.
Interactions Between Plant Viral Nanoparticles (VNPs) and Blood Plasma Proteins, and Their Impact on the VNP In Vivo Fates.
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植物病毒纳米颗粒 (VNP) 和血浆蛋白之间的相互作用及其对 VNP 体内命运的影响。
DOI：
10.1007/978-1-4939-7808-3_38
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Steinmetz,NicoleF
中科院分区：
文献类型：
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作者：
Pitek,AndrzejS;Veliz,FrankA;Jameson,SlaterA;Steinmetz,NicoleF
植物病毒纳米颗粒（VNP）目前正在开发作为新型容器，用于将诊断和治疗货物运送到疾病部位。随着 VNP 变体数量的迅速增加及其在纳米医学中的潜在应用，需要研究它们在血液中获得的特性。已知血浆中存在的生物分子会吸附到纳米材料（包括 VNP）的表面，形成称为蛋白冠的生物界面，该生物界面能够重新编程 VNP 的特性。在这里，我们描述了一些分离和研究 VNP-蛋白冠复合物的通用方法，以评估蛋白冠对靶细胞对 VNP 分子识别和吞噬细胞清除的影响。我们概述了在小鼠模型中体内筛选 VNP 命运的程序，这可能有助于评估不同 VNP 制剂的功效和生物相容性。
Plant viral nanoparticles (VNPs) are currently being developed as novel vessels for delivery of diagnostic and therapeutic cargos to sites of disease. With a rapid increase in the number of VNP variants and their potential applications in nanomedicine, the properties they acquire in the bloodstream need to be investigated. Biomolecules present in plasma are known to adsorb onto the surface of nanomaterials (including VNPs), forming a biointerface called the protein corona, which is capable of reprogramming the properties of VNPs. Here we describe a few general methods to isolate and study the VNP–protein corona complexes, in order to evaluate the impact of protein corona on molecular recognition of VNPs by target cells, and clearance by phagocytes. We outline procedures for in vivo screening of VNP fates in a mouse model, which may be useful for evaluation of efficacy and biocompatibility of different VNP based formulations.