The role of sodium-coupled glucose co-transporter 3 in the satiety effect of portal glucose sensing
The role of sodium-coupled glucose co-transporter 3 in the satiety effect of portal glucose sensing
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DOI：
10.1016/j.molmet.2012.11.003
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发表时间：
2013-02-01
影响因子：
8.1
通讯作者：
Mithieux, Gilles
中科院分区：
文献类型：
--
作者：
Delaere, Fabien;Duchampt, Adeline;Mithieux, Gilles
门静脉葡萄糖传感器检测血糖的变化，以诱导影响食物摄入和葡萄糖稳态的神经信号。先前使用高葡萄糖输注的实验表明涉及葡萄糖转运蛋白2（GLUT 2）的代谢传感。在这里，我们评估了用于检测低葡萄糖通量的信号和候选分子的传入途径。肝总分支迷走神经切断术并不能消除门静脉葡萄糖的厌食作用，表明背侧传输。GLUT 2-null小鼠减少其食物摄入，以响应由富含蛋白质的饮食引发的门静脉葡萄糖信号。Trpm 5缺失小鼠和甜味剂门静脉输注的类似反应也排除了糖味觉受体。相反，α-甲基葡萄糖而不是3-0-甲基葡萄糖的输注减少了食物摄入，而根皮苷阻止了葡萄糖的作用。这表明通过在汇管区表达的SGLT 3进行感知。从这些结果中，我们提出了一个微调的双重机制门静脉葡萄糖传感，响应不同的生理条件。(C)2012 Elsevier GmbH.根据CC BY-NC-ND许可证开放访问。
Portal vein glucose sensors detect variations in glycemia to induce a nervous signal that influences food intake and glucose homeostasis. Previous experiments using high infusions of glucose suggested a metabolic sensing involving glucose transporter 2 (GLUT2). Here we evaluated the afferent route for the signal and candidate molecules for detecting low glucose fluxes. Common hepatic branch vagotomy did not abolish the anorectic effect of portal glucose, indicating dorsal transmission. GLUT2-null mice reduced their food intake in response to portal glucose signal initiated by protein-enriched diet. A similar response of Trpm5-null mice and portal infusions of sweeteners also excluded sugar taste receptors. Conversely, infusions of alpha-methylglucose, but not 3-0-methylglucose, decreased food intake, while phlorizin prevented the effect of glucose. This suggested sensing through SGLT3, which was expressed in the portal area. From these results we propose a finely tuned dual mechanism for portal glucose sensing that responds to different physiological conditions. (C) 2012 Elsevier GmbH. Open access under CC BY-NC-ND license.