Multilocus sequence typing suggests the chytrid pathogen of amphibians is a recently emerged clone
Multilocus sequence typing suggests the chytrid pathogen of amphibians is a recently emerged clone
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DOI：
10.1046/j.1365-294x.2003.01732.x
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发表时间：
2003-02-01
影响因子：
4.9
通讯作者：
Longcore, JE
中科院分区：
文献类型：
--
作者：
Morehouse, EA;James, TY;Longcore, JE
棘霉病是最近发现的一种与全球蛙类数量下降有关的真菌疾病。虽然这种真菌被认为是一种新的病原体，但人们对其种群遗传学知之甚少，包括当前疫情的起源以及这与真菌的传播能力之间的关系。在这项研究中，我们使用多位点序列分型来检测来自北美、非洲和澳大利亚的35个真菌菌株的遗传多样性和亲缘关系。在10个基因座(5918个碱基)中，只检测到5个变异核苷酸位置。这种低水平的遗传变异与树枝杆菌作为一种新近出现的病原体的描述是一致的。两个基因座杂合基因频率固定(即100%)或接近固定，表明树莓为二倍体，主要以无性系繁殖。与缺乏核苷酸多态相反，多个菌株的电泳核型分析显示了许多染色体长度多态。
Chytridiomycosis is a recently identified fungal disease associated with global population declines of frogs. Although the fungus, Batrachochytrium dendrobatidis , is considered an emerging pathogen, little is known about its population genetics, including the origin of the current epidemic and how this relates to the dispersal ability of the fungus. In this study, we use multilocus sequence typing to examine genetic diversity and relationships among 35 fungal strains from North America, Africa and Australia. Only five variable nucleotide positions were detected among 10 loci (5918 bp). This low level of genetic variation is consistent with the description of B. dendrobatidis as a recently emerged disease agent. Fixed (i.e. 100%) or nearly fixed frequencies of heterozygous genotypes at two loci suggested that B. dendrobatidis is diploid and primarily reproduces clonally. In contrast to the lack of nucleotide polymorphism, electrophoretic karyotyping of multiple strains demonstrated a number of chromosome length polymorphisms.