Bioimaging of botulinum toxin and hyaluronate hydrogels using zwitterionic near-infrared fluorophores.
Bioimaging of botulinum toxin and hyaluronate hydrogels using zwitterionic near-infrared fluorophores.
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DOI：
10.1186/s40824-017-0102-x
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发表时间：
2017
影响因子：
11.3
通讯作者：
Hahn SK
中科院分区：
文献类型：
--
作者：
Kim KS;Kim YS;Bao K;Wada H;Choi HS;Hahn SK
注射肉毒杆菌毒素（BTX）以减少面部皱纹是最常见的整形外科手术之一。生物相容性水凝胶注入BTX以有效增强组织。然而，很难确定有效组织填充的注射间隔。将BTX和透明质酸盐（HA）水凝胶用两性离子（ZW）近红外（NIR）荧光团标记，并在注射至BALB/c裸鼠后可视化3周。通过常规EDC/NHS化学成功地制备了BTX-ZW缀合物和二氨基己烷（DAH）-HA-ZW水凝胶。使用NIR荧光成像，我们证实注射后3周约10%的BTX-ZW缀合物和50%的DAH-HA-ZW水凝胶保留。这种使用不可见NIR荧光的生物成像技术可用于各种生物医学应用。
The injection of botulinum toxin (BTX) to reduce facial wrinkles is one of the most frequently performed plastic surgery procedures. The biocompatible hydrogels are injected with BTX for effective tissue augmentation. However, it is difficult to determine the interval of injection for effective tissue augmentation. BTX and hyaluronate (HA) hydrogels were labeled with zwitterionic (ZW) near-infrared (NIR) fluorophores and visualized for 3 weeks after injection to BALB/c nude mice. BTX-ZW conjugates and diaminohexane (DAH)-HA-ZW hydrogels were successfully prepared by the conventional EDC/NHS chemistry. Using the NIR fluorescence imaging, we confirmed that approximately 10% of BTX-ZW conjugates and 50% of DAH-HA-ZW hydrogels remained 3 weeks post-injection. This bioimaging technique using invisible NIR fluorescence light can be exploited for various biomedical applications.