Avian hairy gene expression identifies a molecular clock linked to vertebrate segmentation and somitogenesis
Avian hairy gene expression identifies a molecular clock linked to vertebrate segmentation and somitogenesis
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DOI：
10.1016/s0092-8674(00)80451-1
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发表时间：
1997-11-28
期刊：
影响因子：
64.5
通讯作者：
Pourquie, O
中科院分区：
文献类型：
--
作者：
Palmeirim, I;Henrique, D;Pourquie, O
我们已经鉴定并表征了c-hairy1，它是果蝇分割基因hairy的鸟类同源物。 c-hairy1 在体节前中胚层中强烈表达，其 mRNA 表现出表达的循环波，其时间周期对应于一个体节的形成时间（90 分钟）。这些波的明显运动是由于 c-hairy1 表达的协调脉冲，而不是细胞沿前后轴的位移，也不是激活信号的传播。相反，有节奏的 c-毛 mRNA 表达是轴旁中胚层的自主特性。这些结果为与轴旁中胚层的分割和体节发生相关的发育时钟提供了分子证据，并支持无脊椎动物和脊椎动物使用的分割机制被保守的可能性。
We have identified and characterized c-hairy1, an avian homolog of the Drosophila segmentation gene, hairy. c-hairy1 is strongly expressed in the presomitic mesoderm, where its mRNA exhibits cyclic waves of expression whose temporal periodicity corresponds to the formation time of one somite (90 min). The apparent movement of these waves is due to coordinated pulses of c-hairy1 expression, not to cell displacement along the anteroposterior axis, nor to propagation of an activating signal. Rather, the rhythmic c-hairy mRNA expression is an autonomous property of the paraxial mesoderm. These results provide molecular evidence for a developmental clock linked to segmentation and somitogenesis of the paraxial mesoderm, and support the possibility that segmentation mechanisms used by invertebrates and vertebrates have been conserved.