Constitutive expression of VEGF, VEGFR-1, and VEGFR-2 in normal eyes.
Constitutive expression of VEGF, VEGFR-1, and VEGFR-2 in normal eyes.
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发表时间：
1999-08
影响因子：
4.4
通讯作者：
Ivana K. Kim;A. Ryan;R. Rohan;S. Amano;Stephanie Agular;Joan W. Miller;A. Adamis
中科院分区：
文献类型：
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作者：
Ivana K. Kim;A. Ryan;R. Rohan;S. Amano;Stephanie Agular;Joan W. Miller;A. Adamis
目的探讨血管内皮生长因子（VEGF）及其高亲和力受体VEGFR-1和VEGFR-2在正常眼组织中的表达。方法猴和大鼠在深度麻醉下手术摘除眼球，立即准备研究。采用原位杂交、北方印迹分析、免疫组织化学、免疫测定和逆转录聚合酶链反应相结合的方法研究了眼VEGF、VEGFR-1和VEGFR-2的表达。结果在猴正常血管化眼组织：结膜、虹膜、视网膜和脉络膜-视网膜色素上皮复合体中检测到稳态VEGF mRNA水平。VEGF 121和VEGF 165是鉴定的主要亚型。VEGF蛋白在结膜、视网膜和脉络膜-视网膜色素上皮复合体中检测到。视网膜VEGFmRNA定位于神经节、内核和视网膜色素上皮细胞层。VEGF蛋白定位于这些相同的层和猴视网膜的视锥细胞。在正常猴虹膜、视网膜和脉络膜-视网膜色素上皮复合体中检测到VEGFR-1和VEGFR-2 mRNA。在猴和大鼠的眼睛中，VEGFR-1和VEGFR-2 mRNA定位于视网膜的内核层。结论VEGF、VEGFR-1和VEGFR-2在正常眼血管化组织中呈组成性表达。
PURPOSE The expression of vascular endothelial growth factor (VEGF) and its high-affinity receptors VEGFR-1 and VEGFR-2 was investigated in normal eyes. METHODS Monkey and rat eyes were surgically removed in animals under deep anesthesia and immediately prepared for study. Ocular VEGF, VEGFR-1, and VEGFR-2 expression was studied using a combination of in situ hybridization, northern blot analysis, immunohistochemistry, immunoassay, and reverse transcription-polymerase chain reaction. RESULTS Steady state VEGF mRNA levels were detected in the normal vascularized ocular tissues of the monkey: the conjunctiva, iris, retina, and the choroid-retinal pigment epithelial complex. VEGF121 and VEGF165 were the major isoforms identified. VEGF protein was detected in the conjunctiva, retina, and the choroid-retinal pigment epithelial complex. Retinal VEGF mRNA localized to the ganglion, inner nuclear, and retinal pigment epithelial cell layers. VEGF protein localized to these same layers and to the cones of monkey retina. VEGFR-1 and VEGFR-2 mRNAs were detected in normal monkey iris, retina, and the choroid-retinal pigment epithelial complex. In both monkey and rat eyes, VEGFR-1 and VEGFR-2 mRNAs were localized to the inner nuclear layer of the retina. CONCLUSIONS VEGF, VEGFR-1, and VEGFR-2 are constitutively expressed in the vascularized tissues of normal eyes.