New role for EMD (emerin), a key inner nuclear membrane protein, as an enhancer of autophagosome formation in the C16-ceramide autophagy pathway
New role for EMD (emerin), a key inner nuclear membrane protein, as an enhancer of autophagosome formation in the C16-ceramide autophagy pathway
复制标题
DOI：
10.4161/auto.28777
复制
发表时间：
2014-07-01
期刊：
影响因子：
13.3
通讯作者：
Fillet, Marianne
中科院分区：
文献类型：
--
作者：
Deroyer, Celine;Renert, Anne-Francoise;Fillet, Marianne
迄今为止，EMD （emerin）的确切作用仍然缺乏描述。在本文中，我们研究了EMD在c16 -神经酰胺自噬途径中的作用。神经酰胺是一种具有生物活性的信号分子，在调节细胞生长、分化或细胞死亡中起着重要作用。然而，它们介导这些途径的机制尚不完全清楚。我们发现c16 -神经酰胺通过PRKACA在其LEM结构域诱导EMD磷酸化。经神经酰胺处理后，磷酸化的EMD与MAP1LC3B结合，导致自噬体形成增加。这些数据提示EMD在结肠癌细胞c16 -神经酰胺自噬途径中作为自噬体形成增强剂的新作用。
To date, precise roles of EMD (emerin) remain poorly described. In this paper, we investigated the role of EMD in the C16-ceramide autophagy pathway. Ceramides are bioactive signaling molecules acting notably in the regulation of cell growth, differentiation, or cell death. However, the mechanisms by which they mediate these pathways are not fully understood. We found that C16-ceramide induces EMD phosphorylation on its LEM domain through PRKACA. Upon ceramide treatment, phosphorylated EMD binds MAP1LC3B leading to an increase of autophagosome formation. These data suggest a new role of EMD as an enhancer of autophagosome formation in the C16-ceramide autophagy pathway in colon cancer cells.