Bioanalytical and chemical sensors using living taste, olfactory, and neural cells and tissues: a short review.
Bioanalytical and chemical sensors using living taste, olfactory, and neural cells and tissues: a short review.
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DOI：
10.1039/c5an01288k
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发表时间：
2015-10
期刊：
影响因子：
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通讯作者：
Chunsheng Wu;P. Lillehoj;Ping Wang
中科院分区：
文献类型：
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作者：
Chunsheng Wu;P. Lillehoj;Ping Wang
利用活组织和活细胞的生物传感器作为化学传感和生化分析的功能器件最近受到了极大的关注。这些设备将生物组件(即单细胞、细胞网络、组织)与基于微电子机械系统(MEMS)的传感器和换能器集成在一起。来自天然和生物工程来源的各种类型的细胞和组织被用作识别和传感元件，通常具有高灵敏度和特异性的特点。本文综述了基于组织和细胞的生物传感平台的研究现状，重点介绍了味觉、嗅觉、神经细胞和组织的生物传感平台。其中许多器件采用独特的集成策略和基于敏感传感器的传感方案，包括微电极阵列(MEA)、场效应晶体管(FET)和光寻址电位传感器(LAPS)。几个小组已经将这些混合生物传感器与微流控技术结合在一起，这提供了样本量小和增强的自动化的额外好处。虽然由于活的生物成分的稳定性有限，这项技术目前仅限于实验室环境，但加强其稳定性的进一步研究将使这些设备能够在现场和临床环境中使用。
Biosensors utilizing living tissues and cells have recently gained significant attention as functional devices for chemical sensing and biochemical analysis. These devices integrate biological components (i.e. single cells, cell networks, tissues) with micro-electro-mechanical systems (MEMS)-based sensors and transducers. Various types of cells and tissues derived from natural and bioengineered sources have been used as recognition and sensing elements, which are generally characterized by high sensitivity and specificity. This review summarizes the state of the art in tissue- and cell-based biosensing platforms with an emphasis on those using taste, olfactory, and neural cells and tissues. Many of these devices employ unique integration strategies and sensing schemes based on sensitive transducers including microelectrode arrays (MEAs), field effect transistors (FETs), and light-addressable potentiometric sensors (LAPSs). Several groups have coupled these hybrid biosensors with microfluidics which offers added benefits of small sample volumes and enhanced automation. While this technology is currently limited to lab settings due to the limited stability of living biological components, further research to enhance their robustness will enable these devices to be employed in field and clinical settings.