Distal-less and other homeobox genes in the development of the dentition.
Distal-less and other homeobox genes in the development of the dentition.
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牙列发育中的远端少基因和其他同源盒基因。
DOI：
10.1002/jez.1402700306
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Takashita,K
中科院分区：
文献类型：
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作者：
Weiss,KM;Bollekens,J;Ruddle,FH;Takashita,K
哺乳动物的牙齿是通过不同胚胎起源的两个组织层之间的相互作用而发育的。许多转录因子以及Msx类同源异型盒基因的两个成员已被证明参与哺乳动物牙齿的组织发生。这提出了其他同源异型盒基因可能参与牙齿形态发生的可能性。我们使用18类同源盒基因的简并引物通过聚合酶链反应扩增了三个不同胎龄的小鼠牙胚cDNA。已经分离出几类成员，包括Msx基因、两个Dlx基因和Dbx、MHox、Mox 2A基因。其中一个Dlx基因Dlx-7此前从未在哺乳动物中报道过，本文提供了其cDNA序列的一些细节。这项工作加上其他研究人员的工作表明，至少有六个Dlx基因在发育中的牙齿或第一鳃弓中表达，这表明这些基因可能参与指定牙齿内或牙齿之间的复杂性。简并引物的筛选方法是一种成功的方法，以确定新的以及以前已知的调控基因在发育中的牙齿胚胎表达。© Wiley利斯公司
The mammalian tooth develops through an interaction between two tissue layers of different embryologic origin. A number of transcription factors and as well as two members of theMsxclass of homeobox genes have been shown to be involved in the histogenesis of the mammalian tooth. This raised the possibility that other homeobox genes might be involved in dental morphogenesis. We have amplified mouse tooth germ cDNA from three different gestational ages by the polymerase chain reaction with degenerate primers for 18 classes of homeobox genes. Members of several classes have been isolated, including the Msx genes, two Dlx genes, and the Dbx, MHox, Mox2A genes. One of the Dlx genes, Dlx‐7, had not previously been reported in mammals, and some details are presented of its cDNA sequence. This work plus that of other investigators has shown that at least six Dlx genes are expressed in developing teeth or in first branchial arches, suggesting the possibility that these genes are involved in specifying complexity within or between teeth. The screening approach with degenerate primers is a successful way to identify new as well as previously known regulatory genes expressed in developing tooth embryos. © Wiley‐Liss, Inc.