Hepatitis E virus in rats, Los Angeles, California, USA.
Hepatitis E virus in rats, Los Angeles, California, USA.
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DOI：
10.3201/eid1712.110482
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发表时间：
2011-12
影响因子：
11.8
通讯作者：
Emerson SU
中科院分区：
文献类型：
--
作者：
Purcell RH;Engle RE;Rood MP;Kabrane-Lazizi Y;Nguyen HT;Govindarajan S;St Claire M;Emerson SU
这种病毒不太可能是人畜共患病的威胁。老鼠在人类戊型肝炎病毒(HEV)感染中的作用仍然存在争议。最近从德国的老鼠身上分离出了一种基因独特的HEV，并对其基因组进行了测序。我们已经从美国加利福尼亚州洛杉矶的城市老鼠身上分离出了一种基因相似的HEV，并表征了它感染实验室老鼠和非人类灵长类动物的能力。从≈抗体阳性率为80%的134只野鼠血清标本中分离到2株戊型肝炎病毒。病毒可以传播给血清阴性的Spraogue-Dawley大鼠，但传播不稳定，感染的程度和持续时间不强。裸鼠的病毒血症较高。血清学分析和逆转录聚合酶链式反应在检测感染方面具有较高的敏感性。洛杉矶病毒的序列与来自德国的分离株几乎完全相同。大鼠戊型肝炎病毒不会传播给恒河猴，这表明它不是人类感染源。
This virus is unlikely to be a zoonotic threat. The role of rats in human hepatitis E virus (HEV) infections remains controversial. A genetically distinct HEV was recently isolated from rats in Germany, and its genome was sequenced. We have isolated a genetically similar HEV from urban rats in Los Angeles, California, USA, and characterized its ability to infect laboratory rats and nonhuman primates. Two strains of HEV were isolated from serum samples of 134 wild rats that had a seroprevalence of antibodies against HEV of ≈80%. Virus was transmissible to seronegative Sprague-Dawley rats, but transmission was spotty and magnitude and duration of infection were not robust. Viremia was higher in nude rats. Serologic analysis and reverse transcription PCR were comparably sensitive in detecting infection. The sequence of the Los Angeles virus was virtually identical to that of isolates from Germany. Rat HEV was not transmissible to rhesus monkeys, suggesting that it is not a source of human infection.