BmCyclin B and BmCyclin B3 are required for cell cycle progression in the silkworm, Bombyx mori
BmCyclin B and BmCyclin B3 are required for cell cycle progression in the silkworm, Bombyx mori
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BmCyclin B 和 BmCyclin B3 是家蚕 Bombyx mori 细胞周期进程所必需的
DOI：
10.1007/s11427-013-4459-3
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发表时间：
2013-04-01
影响因子：
9.1
通讯作者：
Lu Cheng
中科院分区：
文献类型：
--
作者：
Pan MinHui;Hong KaiLi;Lu Cheng
细胞周期蛋白B是细胞周期G2期向M期转变的重要调节因子。家蚕基因组数据库显示家蚕中存在两个Cyclin B基因，BmCyclin B和BmCyclin B3。利用家蚕EST数据克隆了细胞周期蛋白B3（EU 074796）基因。其cDNA全长为1665 bp，开放阅读框（ORF）为1536 bp，由7个外显子和6个内含子组成。BmCyclin B3基因编码511个氨基酸，预测分子量为57.8 kD，等电点为9.18。该蛋白含有一个蛋白质损伤盒和两个细胞周期蛋白盒。RNA干扰介导的BmCyclin B和BmCyclin B3表达的减少诱导BmN-SWU 1细胞的细胞周期停滞在G2或M期，从而抑制细胞增殖。这些结果表明，BmCyclin B和BmCyclin B3是完成家蚕细胞周期所必需的。
Cyclin B is an important regulator of the cell cycle G2 to M phase transition. The silkworm genomic database shows that there are two Cyclin B genes in the silkworm (Bombyx mori), BmCyclin B and BmCyclin B3. Using silkworm EST data, the cyclin B3 (EU074796) gene was cloned. Its complete cDNA was 1665 bp with an ORF of 1536 bp derived from seven exons and six introns. The BmCyclin B3 gene encodes 511 amino acids, and the predicted molecular weight is 57.8 kD with an isoelectric point of 9.18. The protein contains one protein damage box and two cyclin boxes. RNA interference-mediated reduction of BmCyclin B and BmCyclin B3 expression induced cell cycle arrest in G2 or M phase in BmN-SWU1 cells, thus inhibiting cell proliferation. These results suggest that BmCyclin B and BmCyclin B3 are necessary for completing the cell cycle in silkworm cells.