The effects of freezing skin on transdermal drug penetration kinetics
The effects of freezing skin on transdermal drug penetration kinetics
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DOI：
10.1111/j.1365-2885.2007.00879.x
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发表时间：
2007-10-01
影响因子：
1.3
通讯作者：
Mills, P. C.
中科院分区：
文献类型：
--
作者：
Ahlstrom, L. A.;Cross, S. E.;Mills, P. C.
本研究考察了冷冻犬皮对氢化可的松渗透动力学的影响。从三只狗的皮肤样本开始进行体外渗透研究，从皮肤采集当天开始（新鲜皮肤），并在-20℃冷冻1、4、8和12个月后再次进行渗透研究。对实验数据进行平均后发现，氢化可的松通过任何时间的皮肤冷冻的伪稳态流量（J(ss)）显著（P < 0.023）大于通过新鲜皮肤的准稳态流量（J(ss)），且冷冻时间长度与δ J（ss）呈正相关（P < 0.007）。对于所有的狗，计算氢化可的松穿过冷冻皮肤的滞后时间（t(lag)）与新鲜皮肤相比显著（P < 0.029）缩短。然而，氢化可的松通过新鲜和冷冻狗皮肤的渗透曲线形状相似，组织学检查各组之间没有差异。本研究结果表明，在-20℃下冷冻狗皮肤可以显著增加氢化可的松的体外透皮渗透，并且这种增强的程度随冷冻时间的延长而增加。
This study investigated the effects of freezing canine skin on the penetration kinetics of hydrocortisone. Skin samples from three dogs were used for in vitro penetration studies commencing on the day of skin collection (fresh skin) and again after freezing at -20 degrees C for 1, 4, 8 and 12 months. When the data from the dogs was averaged, the pseudo-steady-state flux (J(ss)) of hydrocortisone through skin frozen for any duration was significantly (P < 0.023) greater than through fresh skin and there was a positive relationship (P < 0.007) between the length of freezing and Delta J(ss). For all dogs, the lag times (t(lag)) calculated for hydrocortisone penetration were significantly (P < 0.029) shorter through skin that had been frozen, compared with fresh skin. However, the shapes of the permeation profiles of hydrocortisone appeared similar through the fresh and frozen dog skins and no differences were detected between the groups on histological examination. The results of this study have shown that freezing dog skin at -20 degrees C can significantly increase the transdermal penetration of hydrocortisone in vitro, and that the extent of this enhancement can increase with duration of freezing.