Immunoactivation enhances the concentration of luteinizing hormone-releasing hormone peptide and its gene expression in human peripheral T-lymphocytes.
Immunoactivation enhances the concentration of luteinizing hormone-releasing hormone peptide and its gene expression in human peripheral T-lymphocytes.
复制标题
DOI：
10.1210/endo.133.1.8319570
复制
发表时间：
1993-07
期刊：
影响因子：
4.8
通讯作者：
N. Azad;N. L. Paglia;K. A. Jurgens;L. Kirsteins;N. Emanuele;M. R. Kelley;A. Lawrence;N. Mohagheghpour
中科院分区：
文献类型：
--
作者：
N. Azad;N. L. Paglia;K. A. Jurgens;L. Kirsteins;N. Emanuele;M. R. Kelley;A. Lawrence;N. Mohagheghpour
当我们报道大鼠脾和胸腺淋巴细胞中存在免疫活性和生物活性的LHRH及其mRNA时，建议LHRH的免疫调节作用。本文报道了人外周血T细胞及其亚群CD_（4+）和CD_（8+）含有免疫活性和生物活性的LHRH。此外，委员会认为，通过RIA对植物血凝素（PHA）活化的T细胞裂解物的LHRH分析表明，PHA活化的T细胞中LHRH的平均浓度在培养24小时后从45 +/-4.5增加到64 +/-7 pg/10（6）细胞，并且从47 +/-3.6增加到117 +/-11.8 pg/10（6）细胞48 h后，与对照组比较，差异有显著性（P < 0.01）。在PHA激活的细胞中LHRH浓度在培养的最后24小时从64 +/- 7增加到117 +/- 11.8 pg/10（6）细胞（P < 0.001），而在单独的培养基中T细胞中LHRH的平均浓度没有变化。在初步研究中，我们发现新鲜T细胞含有20 +/- 1.4 pg pro-LHRH/10（6）细胞，PHA刺激增加pro-LHRH含量，类似于LHRH的增加。与未分级的T细胞一样，在CD 4+和CD 8 + T细胞中注意到PHA诱导的细胞内LHRH的显著时间依赖性增强。从淋巴细胞中提取的RNA进行逆转录聚合酶链反应分析，使用LHRH和组蛋白-3.3，引物，后者作为内部对照。聚合酶链反应生成的数据表明，在培养的，但非PHA刺激（休息），细胞的LHRH mRNA的相对量显着减少5-24小时之间，但恢复48小时的培养。PHA刺激的细胞中LHRH mRNA的相对量显示出显着不同的模式。PHA激活的细胞中LHRH信息表达在培养5 h时略有增加，在培养24 h时达到最大刺激，但在培养48 h时下降。PHA激活诱导的LHRH肽在5和24 h的细胞内积累的时间依赖性增强伴随着增加的LHRH信息。然而，48 h时LHRH肽浓度的增加与LHRH信息表达的减少相一致。PHA激活细胞的总蛋白质合成数据显示蛋白质合成的逐步增加，这种模式完全类似于LHRH细胞含量的变化。（400字处截断摘要）
An immunomodulatory role for LHRH was suggested when we reported the presence of immunoactive and bioactive LHRH and its mRNA in rat splenic and thymic lymphocytes. In this paper we report that human peripheral T-cells as well as its subsets CD4+ and CD8+ contained immunoactive and bioactive LHRH. Furthermore, analysis of phytohemagglutinin (PHA)-activated T-cell lysates for LHRH by RIA demonstrated that the mean concentration of LHRH in PHA-activated T-cells increased from 45 +/- 4.5 to 64 +/- 7 pg/10(6) cells after 24 h of culture and from 47 +/- 3.6 to 117 +/- 11.8 pg/10(6) cells (P < 0.01) after 48 h. While the LHRH concentration in PHA-activated cells increased over the last 24 h of culture h from 64 +/- 7 to 117 +/- 11.8 pg/10(6) cells (P < 0.001), there was no change in mean concentration of LHRH in T-cells kept in medium alone. In a preliminary study we found that fresh T-cells contain 20 +/- 1.4 pg pro-LHRH/10(6) cells, and PHA stimulation increased the pro-LHRH content similar to the increase in LHRH. As with unfractionated T-cells, a significant PHA-induced time-dependent enhancement of intracellular LHRH was noted in CD4+ and CD8+ T-cells. RNA extracted from lymphocytes was subjected to reverse transcription-polymerase chain reaction analysis using LHRH and histone-3.3, primers, the latter as an internal control. The polymerase chain reaction-generated data demonstrated that the relative amount of LHRH mRNA in cultured, but non-PHA-stimulated (resting), cells diminished dramatically between 5-24 h, but recovered by 48 h of culture. The relative amount of LHRH mRNA in PHA-stimulated cells revealed a markedly different pattern. LHRH message expression in PHA-activated cells increased slightly at 5 h of culture and was maximally stimulated by 24 h, but declined by 48 h of culture. The PHA activation-induced time-dependent enhancement of intracellular accumulation of LHRH peptide at 5 and 24 h was accompanied by increased LHRH message. However, the increased concentration of LHRH peptide at 48 h coincided with decreased LHRH message expression. The data from total protein synthesis in PHA-activated cells showed a progressive increase in protein synthesis, a pattern entirely similar to the changes in the cell content of LHRH.(ABSTRACT TRUNCATED AT 400 WORDS)