GLP-1 Secretion in Response to Oral and Luminal Palatinose (Isomaltulose) in Rats
GLP-1 Secretion in Response to Oral and Luminal Palatinose (Isomaltulose) in Rats
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DOI：
10.3177/jnsv.57.30
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发表时间：
2011-02-01
影响因子：
1.6
通讯作者：
Hara, Hiroshi
中科院分区：
文献类型：
--
作者：
Hira, Tohru;Muramatsu, Maya;Hara, Hiroshi
帕拉金糖（异麦芽酮糖）。一种缓慢消化的二糖，用作非致龋糖和几种食物中的蔗糖替代物。由于帕拉金糖能够降低餐后血糖，它可能是治疗糖代谢受损的有益药物。胰高血糖素样肽-1（GLP-1）通过增强胰腺β细胞功能来改善胰腺炎。GLP-1的分泌受到糖的刺激，包括葡萄糖和人工甜味剂。在这项研究中，我们研究了帕拉金糖是否在体内和体外诱导GLP-1分泌。首先，清醒大鼠口服帕拉金糖或蔗糖后，测定门脉GLP-1和葡萄糖。其次，在麻醉大鼠中，在空肠或回肠施用每种糖后测量门静脉GLP-1和葡萄糖。最后，将GLUTag（一种产生GLP-1的鼠细胞系）暴露于几种糖（包括帕拉金糖和蔗糖），以观察这些糖对GLP-1分泌的直接影响。与蔗糖相比，帕拉金糖在清醒大鼠中经口给药时增强门脉GLP-1水平。帕拉金糖和蔗糖诱导门静脉GLP-1显着增加后，空肠或回肠给药的每种糖在麻醉大鼠。回肠给药比空肠给药引发更大的反应。在每个实验中，蔗糖处理的大鼠中的谷胱甘肽反应高于帕拉金糖处理的大鼠。在GLUTag细胞中，葡萄糖诱导GLP-1分泌显着增加，但蔗糖和帕拉金糖都没有影响。这些数据表明鲁米那帕拉金糖诱导大鼠的GLP-1分泌。然而，GLP-1的分泌可能主要由管腔中释放的葡萄糖而不是帕拉金糖本身触发。
Palatinose (isomaltulose). a slowly digested disaccharide, is used as a noncariogenic sugar and as a sucrose substitute in several foods. Because of its ability to lower postprandial glycemia, palatinose may be beneficial as a treatment for impaired glucose metabolism. Glucagon-like peptide-1 (GLP-1) improves glycemia via enhancing pancreatic beta-cell functions. The secretion of GLP-1 is stimulated by sugars, including glucose and artificial sweeteners. In this study, we examined whether palatinose induced GLP-1 secretion in vivo and in vitro. Firstly, portal GLP-1 and glucose were measured after oral administration of palatinose or sucrose in conscious rats. Secondly, portal GLP-1 and glucose were measured after jejunal or ileal administration of each sugar in anesthetized rats. Finally, GLUTag, a murine GLP-1-producing cell line, was exposed to several sugars, including palatinose and sucrose, to observe the direct effect of these sugars on GLP-1 secretion. Compared with sucrose, palatinose enhanced portal GLP-1 level when administered orally in conscious rats. Both palatinose and sucrose induced a significant increase in portal GLP-1 after jejunal or ileal administration of each sugar in anesthetized rats. Ileal administration triggered a greater response than did jejunal administration. Glycemic responses were higher in sucrose-treated rats than in palatinose-treated rats in every experiment. In GLUTag cells, glucose induced a significant increase in GLP-1 secretion, but neither sucrose nor palatinose had an effect. These data demonstrate that luminal palatinose induces GLP-1 secretion in rats. However, it is likely that GLP-1 secretion is triggered mainly by glucose released in the lumen rather than by palatinose itself.