The Tn3 beta-lactamase gene acts as a hotspot for meiotic recombination in yeast.
The Tn3 beta-lactamase gene acts as a hotspot for meiotic recombination in yeast.
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Tn3 β-内酰胺酶基因是酵母减数分裂重组的热点。
DOI：
10.1093/genetics/127.1.39
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发表时间：
1991
期刊：
影响因子：
3.3
通讯作者：
Petes,TD
中科院分区：
文献类型：
--
作者：
Stapleton,A;Petes,TD
尽管遗传距离通常被认为与物理距离成正比，但在许多遗传系统中已经描述了减数分裂重组水平异常高(热点)或低(冷点)的染色体区域。一般说来，造成这些影响的DNA序列尚未确定。我们报道了细菌转座子Tn3的β-内酰胺酶(AMPR)基因5‘端插入酿酒酵母染色体后的减数分裂重组热点。当这些序列是纯合子时，交换和基因转换都是局部刺激的。与普通的酵母DNA序列相比，β-内酰胺酶基因的5‘端对于交叉大约要“热”100倍。
Although genetic distances are often assumed to be proportional to physical distances, chromosomal regions with unusually high (hotspots) or low (coldspots) levels of meiotic recombination have been described in a number of genetic systems. In general, the DNA sequences responsible for these effects have not been determined. We report that the 5' region of the beta-lactamase (ampR) gene of the bacterial transposon Tn3 is a hotspot for meiotic recombination when inserted into the chromosomes of the yeast Saccharomyces cerevisiae. When these sequences are homozygous, both crossing over and gene conversion are locally stimulated. The 5' end of the beta-lactamase gene is about 100-fold "hotter" for crossovers than an average yeast DNA sequence.