Silencing GTSE-1 expression inhibits proliferation and invasion of hepatocellular carcinoma cells.
Silencing GTSE-1 expression inhibits proliferation and invasion of hepatocellular carcinoma cells.
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沉默GTSE-1表达抑制肝癌细胞增殖和侵袭
DOI：
10.1007/s10565-016-9327-z
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发表时间：
2016-08
影响因子：
6.1
通讯作者：
Ye Q
中科院分区：
文献类型：
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作者：
Guo L;Zhang S;Zhang B;Chen W;Li X;Zhang W;Zhou C;Zhang J;Ren N;Ye Q
G2和S期表达的-1（GTSE 1）最近被报道在几种类型的人类癌症中上调，基于负调节p53表达。然而，其在肝细胞癌（HCC）中的表达和功能作用仍不清楚。在这项研究中，观察到GTSE 1在HCC标本和细胞系中在信使RNA（mRNA）和蛋白质水平上高度表达。此外，GTSE 1高表达与肿瘤大小、静脉浸润、晚期肿瘤分期和总生存期短呈正相关。此外，我们建立了稳定的GTSE 1敲减肝癌细胞系，以探讨GTSE 1沉默对肝癌体外生长和侵袭的影响。在确定GTSE 1调节细胞增殖和侵袭的途径时，发现GTSE 1沉默抑制AKT磷酸化并下调细胞周期相关蛋白。此外，GTSE 1下调降低了异种移植物的生长。总之，这些结果首次表明GTSE 1过表达参与了HCC的进展，增强了HCC细胞的增殖和促进细胞侵袭。
G2 and S phase-expressed-1 (GTSE1) was recently reported to upregulate in several types of human cancer, based on negatively regulate p53 expression. However, its expression and functional roles in hepatocellular carcinoma (HCC) remain unknown. In this study, GTSE1 was observed to be highly expressed in HCC specimens and cell lines both at messenger RNA (mRNA) and protein levels. Furthermore, high GTSE1 expression was positively associated with tumor size, venous invasion, advanced tumor stage, and short overall survival. Moreover, we generated stable GTSE1 knockdown HCC cell lines to explore the effects of GTSE1 silencing on the growth and invasion of HCC in vitro. In determining the pathway through which GTSE1 regulated cell proliferation and invasion, GTSE1 silencing was found to inhibit AKT phosphorylation and downregulated cell cycle-related protein. In addition, GTSE1 downregulation decreased the growth of xenografts. In conclusion, these results indicated for the first time that overexpression of GTSE1 was involved in the progress of HCC, enhancing proliferation and promoting cell invasion in HCC cells.