Towards Mapping of the Human Brain N-Glycome with Standardized Graphitic Carbon Chromatography.
Towards Mapping of the Human Brain N-Glycome with Standardized Graphitic Carbon Chromatography.
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用标准化石墨碳色谱法测定人脑n -糖苷。
DOI：
10.3390/biom12010085
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发表时间：
2022-01-06
期刊：
影响因子：
5.5
通讯作者：
Altmann F
中科院分区：
文献类型：
--
作者：
Helm J;Hirtler L;Altmann F
众所周知，脑n -糖对许多生物学功能至关重要，包括它与神经元疾病的关系。尽管最近对脑n -聚糖进行了大量的结构研究，但全面的同分异构体特异性结构分析仍然没有实现，正如最近发现的半乳糖化的二分GlcNAc的新结构所表明的那样。在这里，我们提出了一个详细的，基于标准化多孔石墨碳(PGC)-LC-MS/MS的人脑n -糖的异构体特异性分析。为了实现这一目标，我们生物合成了通常发生在脑n -糖苷中的取代聚糖，并获得了它们的标准化保留时间。将这些值与人脑中性和脱氮化n -聚糖组分的标准保留时间进行比较，可以确定大多数主要峰的异构体特异性分配。发现天然中性和脱盐聚糖之间的聚糖结构存在深刻差异。中性和唾液化的n -聚糖来源于不同的生物合成途径，是生物合成的最终产物，而不仅仅是部分加工的中间体。对结构糖组学的关注定义了人脑n -聚糖的结构，其中包括含有HNK-1的聚糖，一种分二的唾液乳糖，以及在同一臂上具有焦点和n -乙酰半乳糖的结构，即所谓的LDNF表位，通常与寄生虫有关。
The brain N-glycome is known to be crucial for many biological functions, including its involvement in neuronal diseases. Although large structural studies of brain N-glycans were recently carried out, a comprehensive isomer-specific structural analysis has still not been achieved, as indicated by the recent discovery of novel structures with galactosylated bisecting GlcNAc. Here, we present a detailed, isomer-specific analysis of the human brain N-glycome based on standardized porous graphitic carbon (PGC)-LC-MS/MS. To achieve this goal, we biosynthesized glycans with substitutions typically occurring in the brain N-glycome and acquired their normalized retention times. Comparison of these values with the standardized retention times of neutral and desialylated N-glycan fractions of the human brain led to unambiguous isomer specific assignment of most major peaks. Profound differences in the glycan structures between naturally neutral and desialylated glycans were found. The neutral and sialylated N-glycans derive from diverging biosynthetic pathways and are biosynthetically finished end products, rather than just partially processed intermediates. The focus on structural glycomics defined the structure of human brain N-glycans, amongst these are HNK-1 containing glycans, a bisecting sialyl-lactose and structures with fucose and N-acetylgalactosamine on the same arm, the so-called LDNF epitope often associated with parasitic worms.
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DOI：
10.1083/jcb.121.6.1409
发表时间：
1993-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Horstkorte R;Schachner M;Magyar JP;Vorherr T;Schmitz B
通讯作者：
Schmitz B
DOI：
10.3390/molecules26175176
发表时间：
2021-08-26
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Kawade H;Morise J;Mishra SK;Tsujioka S;Oka S;Kizuka Y
通讯作者：
Kizuka Y
影响因子：
4.3
作者：
Akasaka-Manya, Keiko;Manya, Hiroshi;Endo, Tamao
通讯作者：
Endo, Tamao
影响因子：
4.3
作者：
CHAN A L;MORRIS H R;DELL A
通讯作者：
DELL A
DOI：
10.1016/j.mcpro.2021.100130
发表时间：
2021
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Barboza M;Solakyildirim K;Knotts TA;Luke J;Gareau MG;Raybould HE;Lebrilla CB
通讯作者：
Lebrilla CB