Establishment of Cre/LoxP recombination system in transgenic rats.
Establishment of Cre/LoxP recombination system in transgenic rats.
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DOI：
10.1016/j.bbrc.2004.04.204
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发表时间：
2004-07
影响因子：
3.1
通讯作者：
Yuki Sato;H. Endo;T. Ajiki;Y. Hakamata;T. Okada;T. Murakami;E. Kobayashi
中科院分区：
文献类型：
--
作者：
Yuki Sato;H. Endo;T. Ajiki;Y. Hakamata;T. Okada;T. Murakami;E. Kobayashi
大鼠为包括外科手术在内的生物医学研究提供了重要的动物模型。然而，先进的基因操作在大鼠身上的进展不如在小鼠身上的进展。在这里，我们报道了Cre/LoxP转基因大鼠系统，显示了受精和成年阶段的条件染色体易位，基于导管的腺病毒基因转移的时空基因控制，以及肢体移植中的肌肉融合事件。利用大鼠比小鼠体型更大的优势，这种大鼠系统在评估基因治疗和再生医学的生物医学和治疗意义方面具有潜在的价值。
The rat has offered an important animal model in biomedical research including surgical procedure. However, advanced genetic manipulation has progressed less far in the rat than in the mouse. Here we report the Cre/LoxP transgenic rat system, demonstrating conditional chromosomal translocation both in the fertilization and adult stage, spatio-temporal gene controlling by catheter-based adenoviral gene transfer, and muscular fusion events in the limb transplant. Taking advantage of the larger body size of the rat than the mouse, this rat system provides a potential value to evaluate biomedical and therapeutic significance for gene therapy and regenerative medicine.