Sub-chronic carbendazim exposure induces hepatic glycolipid metabolism disorder accompanied by gut microbiota dysbiosis in adult zebrafish (Daino rerio)
Sub-chronic carbendazim exposure induces hepatic glycolipid metabolism disorder accompanied by gut microbiota dysbiosis in adult zebrafish (Daino rerio)
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DOI：
10.1016/j.scitotenv.2020.140081
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发表时间：
2020-10-15
影响因子：
9.8
通讯作者：
Jin, Yuanxiang
中科院分区：
文献类型：
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作者：
Bao, Zhiwei;Zhao, Yao;Jin, Yuanxiang
多菌灵是一种广谱杀菌剂，在世界范围内广泛应用于植物病害防治。随着CBZ在农业上的应用，在蔬菜和水果中也检测到了CBZ。如今，它甚至已经在水道和室内灰尘中被检测到。然而，CBZ对水生生物的毒性效应受到的关注有限。在这项研究中，雄性成年斑马鱼暴露于0，30和100 μ g/L的CBZ 21天，以评估其对肝脏糖脂代谢的影响。暴露后，体重和身长下降，但条件因子显著增加。CBZ暴露后，斑马鱼肝脏中葡萄糖、丙酮酸、低密度脂蛋白（LDL）和甘油三酯（TG）等生化指标显著降低，丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）显著升高，表明亚慢性CBZ暴露对肝脏功能有影响。此外，与肝脏糖酵解和脂质代谢相关的一些关键基因的相对mRNA水平也发生了显著变化。转录组分析表明，CBZ暴露对斑马鱼肝脏的碳代谢、脂代谢和解毒代谢也有影响。有趣的是，我们还发现厚壁菌门、拟杆菌门、放线菌门、α-变形菌门、γ-变形菌门和疣微菌门在门水平上的数量在肠道中显著减少。对16 S rRNA的V3-V4区域进行测序也表明，根据加权UniFrac距离分析，肠道微生物群组成发生了显著变化。因此，亚慢性CBZ暴露诱导肝代谢紊乱伴随肠道微生物群生态失调的成年雄性斑马鱼。(C)2020 Elsevier B. V.保留所有权利。
Carbendazim (CBZ) as a broad spectrum fungicide is widely used in the whole world to contort plant diseases. With the application of CBZ in the agriculture, it has been detected in vegetables and fruits. Nowadays, it even has been detected in the watercourse and indoor dust. However, the toxic effects of CBZ on aquatic organisms have received limited attention. In this study, male adult zebrafish were exposed at 0, 30 and 100 mu g/L CBZ for 21 days to assess its effects on hepatic glycolipid metabolism. After exposure, the body weight and length decreased, but the condition factor increased significantly. Some hepatic biochemical parameters including the levels of glucose, pyruvate, low density lipoprotein (LDL) and triglyceride (TG) decreased significantly in the liver of zebrafish after exposure with CBZ Two transaminases alanine transaminase (ALT) and aspartate transaminase (AST) also increased significantly, indicating that subchronic CBZ exposure influenced the liver function. Moreover, the relative mRNA levels of some key genes related to the glycolysis and lipid metabolism in the liver also changed significantly. Furthermore, the transcriptome analysis showed that the carbon metabolism, lipid metabolism and detoxification metabolism were also affected in the liver of CBZ exposed zebrafish. Interestingly, we also found the amounts of the Firmicutes, Bacteroidetes, Actinobacteria, alpha-Proteobacteria, gamma-Proteobacteria and Verrucomicrobia at phylum level significantly decreased in the gut. Sequencing V3-V4 region of 16S rRNA also demonstrated gut microbiota composition changed significantly according to weighted UniFrac distance analysis. Consequently, subchronic CBZ exposure induced hepatic metabolic disorder accompanied by gut microbiota dysbiosis in adult male zebrafish. (C) 2020 Elsevier B.V. All rights reserved.