Bm1-Bm5 classification of peripheral blood B cells reveals circulating germinal center founder cells in healthy individuals and disturbance in the B cell subpopulations in patients with primary Sjogren's syndrome
Bm1-Bm5 classification of peripheral blood B cells reveals circulating germinal center founder cells in healthy individuals and disturbance in the B cell subpopulations in patients with primary Sjogren's syndrome
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DOI：
10.4049/jimmunol.167.7.3610
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发表时间：
2001-10-01
影响因子：
4.4
通讯作者：
Thompson, KM
中科院分区：
文献类型：
--
作者：
Bohnhorst, JO;Bjorgan, MB;Thompson, KM
对骨髓和次级淋巴组织中 B 细胞的分析揭示了定义 B 细胞亚群的广泛细胞表面标记，但其中只有少数用于分析外周血 (PB) 中的 B 细胞亚群。我们在此报告通过 CD19、CD38 和 IgD 结合 CD10、CD44、CD77、CD95、CD23、IgM 和 B 细胞记忆标记 CD27 染色来描绘循环 PB B 细胞亚群。自身免疫性疾病患者循环 B 细胞亚群的紊乱证明了这种方法的实用性。在正常 PB 中鉴定出五个成熟 B 细胞 (Bm) 亚群，它们与扁桃体 Bm1、Bm2、早期 Bm5、Bm5 亚群相当，令人惊讶的是，与生发中心 (GC) 创始细胞亚群（Bm2' 和 Bm3 delta -4 delta）相当，表明一些 GC 创始细胞正在循环。没有 PB B 细胞与 Bm3 和 Bm4 GC 细胞相似。值得注意的是，一些具有 CD38(-)IgD(+) 表型的细胞（以前称为幼稚 Bm1 细胞）表达 CD27。因此，CD38(-)IgD(+) 亚群包括初始 Bmi 细胞和 lgD(+) 记忆 B 细胞。这种 B 细胞发育阶段的新分类揭示了与健康供体和类风湿关节炎患者相比，原发性干燥综合征 (pSS) 患者的 B 细胞亚群比例存在紊乱。 pSS 患者在两个激活阶段的 B 细胞百分比显着较高，这可能反映了 B 细胞运输的紊乱和/或 B 细胞分化的改变。这些发现可能对 pSS 具有诊断意义。
Analyses of B cells in the bone marrow and secondary lymphoid tissues have revealed a broad range of cell surface markers defining B cell subpopulations, but only a few of these have been used to analyze B cell subpopulations in peripheral blood (PB). We report here the delineation of circulating PB B cell subpopulations by staining for CD19, CD38, and IgD in combination with CD10, CD44, CD77, CD95, CD23, IgM, and the B cell memory marker CD27. The utility of this approach is shown by the demonstration of disturbances of circulating B cell subpopulations in patients with autoimmune disease. Five mature B cell (Bm) subpopulations were identified in normal PB that were comparable with the tonsillar Bm1, Bm2, early Bm5, Bm5 subpopulations and, surprisingly, to the germinal center (GC) founder cell subpopulation (Bm2' and Bm3 delta -4 delta), suggesting that some GC founder cells are circulating. No PB B cells resembled the Bm3 and Bm4 GC cells. Remarkably, some cells with the CD38(-)IgD(+) phenotype, previously known as naive Bm1 cells, expressed CD27. The CD38(-)IgD(+) subpopulation therefore includes both naive Bmi cells and lgD(+) memory B cells. This new classification of B cell developmental stages reveals disturbances in the proportions of B cell subpopulations in primary Sjogren's syndrome (pSS) patients compared with healthy donors and rheumatoid arthritis patients. Patients with pSS contained a significantly higher percentage of B cells in two activated stages, which might reflect a disturbance in B cell trafficking and/or alteration in B cell differentiation. These findings could be of diagnostic significance for pSS.